食品与发酵工业 >

2026 , Vol. 52 >Issue 5: 101 - 110

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042691

研究报告

复合益生元组合缓解小鼠特应性皮炎并恢复肠道菌群失调

  • 喻喜华 ,
  • 王一 ,
  • 李刘若兰 ,
  • 陈喜生 ,
  • 王宁 ,
  • 王鸿超
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  • 1(国药控股星鲨制药(厦门)有限公司,福建 厦门,361026)
    2(厦门市母婴健康营养产品重点实验室,福建 厦门,361026)
    3(江南大学 食品学院,江苏 无锡,214000)
第一作者:硕士,高级工程师(王鸿超副研究员为通信作者,E-mail:hcwang@jiangnan.edu.cn)

收稿日期: 2025-03-12

  修回日期: 2025-07-14

  网络出版日期: 2026-03-25

收起

A designed prebiotic mixture alleviates atopic dermatitis and restores intestinal dysbiosis in mice

  • YU Xihua ,
  • WANG Yi ,
  • LI Liuruolan ,
  • CHEN Xisheng ,
  • WANG Ning ,
  • WANG Hongchao
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  • 1(Sinopharm Xingsha Pharmaceuticals (Xiamen) Co.Ltd., Xiamen 361026, China)
    2(Xiamen Key Laboratory of Maternal and Infant Health and Nutrition Products, Xiamen 361026, China)
    3(School of Food Science and Technology, Jiangnan University, Wuxi 214000, China)

Received date: 2025-03-12

  Revised date: 2025-07-14

  Online published: 2026-03-25

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摘要

特应性皮炎(atopic dermatitis,AD)是一种普遍的过敏性皮肤疾病。该研究基于前期文献调研,选择了具有免疫调节作用的多种益生元,包括低聚果糖、低聚半乳糖、低聚木糖和菊粉,旨在探究单个益生元及其组合对AD症状的缓解效果。结果显示益生元干预缓解了AD小鼠皮肤病理症状,并伴随着过敏炎症相关的效应细胞浸润减少,血清免疫球蛋白E和皮肤组织中IL-4、IL-5、IL-13和胸腺基质淋巴生成素等炎症因子水平的降低,IFN-γ水平的升高。在所有组别中,4种益生元的组合的施用比单一益生元的施用显示出更优异的效果。此外,通过对小鼠肠道菌群进行测序分析发现,益生元组合改变了肠道菌群结构,提高了菌群多样性,在属水平上显著提高了Angelakisella和Ruminiclostridium 5等潜在有益菌的相对丰度,在操作分类单元水平上显著提高了Erysipelotrichaceae bacterium NYU-BL-F16等的相对丰度。肠道菌群互作网络和关联性分析结果表明,益生元组合干预富集的肠道菌与免疫炎症因子的改变显著相关。综上,混合益生元显示出更好的AD缓解效果,即实现了协同增效,有助于通过改变肠道微生物群结构组成和调节免疫反应来缓解AD,为开发改善AD的新策略提供了充足的科学依据。

关键词： 益生元; 特应性皮炎; 肠道微生物群; 免疫调节; 菌群多样性; 肠道菌互作

本文引用格式

喻喜华 , 王一 , 李刘若兰 , 陈喜生 , 王宁 , 王鸿超 . 复合益生元组合缓解小鼠特应性皮炎并恢复肠道菌群失调[J]. 食品与发酵工业, 2026 , 52(5) : 101 -110 . DOI: 10.13995/j.cnki.11-1802/ts.042691

Abstract

Atopic dermatitis (AD) is a type of allergic skin disorder with high occurrence frequency.In this study, multiple prebiotics with immunomodulatory properties, including fructooligosaccharides (FOS), galactooligosaccharides (GOS), xylooligosaccharides (XOS), and inulin were selected based on literature review, aiming to investigate alleviation effects of individual prebiotics and their mixture on AD symptoms.The results indicated that prebiotic intervention alleviated pathological symptoms of skin in AD mice, accompanied by reduced infiltration of allergy-associated cells, decreased levels of immunoglobulin E (IgE) in serum and inflammatory cytokines, i.e. IL-4, IL-5, IL-13, and thymic stromal lymphopoietin (TSLP) in skin tissue, along with increased levels of IFN-γ in skin tissue.Among all groups, the mixture of four prebiotics demonstrated more efficient alleviation compared to individual prebiotic components.Sequencing analysis on gut microbiota revealed that the prebiotic mixture significantly regulated community structure, increased α-diversity, markedly elevated the relative abundance of potential beneficial genera (e.g., Angelakisella and Ruminiclostridium 5), and enriched abundances of operational taxonomic units such as Erysipelotrichaceae bacterium NYU-BL-F16.Microbial interaction network and correlation analyses demonstrated that enriched gut bacteria in response to prebiotic mixture intervention were significantly associated with alterations in inflammatory-associated indexes.Collectively, the prebiotic mixture exhibited more efficient mitigation against AD, achieving synergistic effects, which would contribute to AD alleviation via routes of regulating gut microbiota and modulating immune aspects of host, and provide scientific basis for development of novel intervention strategy against AD.

Key words： probiotics; atopic dermatitis; gut microbiota; immunomodulation; gut microbiota diversity; microbe-microbe interactions

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