食品与发酵工业 >

2026 , Vol. 52 >Issue 8: 396 - 402

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044876

分析与检测

超分子溶剂的制备及其在蚕蛹粉中黄曲霉毒素快速测定的应用

  • 王东旭 ,
  • 韦铮 ,
  • 王艳艳 ,
  • 杨帆 ,
  • 胡斯蒙
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  • (湖州市食品药品检验研究院,浙江 湖州,313000)
第一作者:博士,高级工程师(通信作者,E-mail:11237122@zju.edu.cn)

收稿日期: 2025-10-20

  修回日期: 2025-11-27

  网络出版日期: 2026-05-07

基金资助

湖州市公益性应用研究项目(2021GZ29)

收起

Preparation and application of supramolecular solvents in determination of aflatoxins in silkworm pupae powder

  • WANG Dongxu ,
  • WEI Zheng ,
  • WANG Yanyan ,
  • YANG Fan ,
  • HU Simeng
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  • (Huzhou Institute for Food and Drug Control, Huzhou 313000, China)

Received date: 2025-10-20

  Revised date: 2025-11-27

  Online published: 2026-05-07

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摘要

基于蚕蛹粉中黄曲霉毒素污染引发的食品安全风险,该研究开发了一种盐析辅助超分子溶剂液液萃取结合超高效液相色谱-质谱同时检测蚕蛹粉中4种黄曲霉毒素高通量检测方法。通过对超分子溶剂比例、用量及盐析剂用量等关键因素进行考察优化,蚕蛹粉样本以正庚醇、四氢呋喃和水形成的超分子溶剂进行萃取,硫酸铵作为盐析助剂分层,提取液加水定容后,采用C18反相色谱柱分离,电喷雾正离子模式检测,外标法定量。最优实验条件下,在0.1～10.0 ng/mL范围内4种黄曲霉毒素线性良好,相关系数均大于等于0.999,检出限为0.03～0.12 μg/kg,定量限为0.1～0.4 μg/kg。3个浓度水平下加标回收率为77.3%～99.2%,精密度为0.4%～5.2%。该方法快捷、绿色、低廉,可满足对蚕蛹粉中4种黄曲霉毒素的快速准确分析要求。

关键词： 蚕蛹粉; 黄曲霉毒素; 超分子溶剂; 超高效液相色谱-质谱

本文引用格式

王东旭 , 韦铮 , 王艳艳 , 杨帆 , 胡斯蒙 . 超分子溶剂的制备及其在蚕蛹粉中黄曲霉毒素快速测定的应用[J]. 食品与发酵工业, 2026 , 52(8) : 396 -402 . DOI: 10.13995/j.cnki.11-1802/ts.044876

Abstract

Based on the food safety risks caused by aflatoxin contamination in silkworm pupa powder, a high-throughput detection method was developed using salting-out assisted supramolecular solvent liquid-liquid extraction combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for simultaneous determination of four aflatoxins in silkworm pupa powder.After optimizing the key factors such as the composition and amount of supramolecular solvent,and the amount of salt,silkworm pupae powder samples were first subjected to extraction with a 1-heptanol/tetrahydrofuran/water-based supramolecular solvent, then salted out by sodium sulfate, separated on a C18 column, and finally determined by electrospray tandem mass spectrometry in positive ion and multiple reaction monitoring (MRM) mode, followed by external standard quantification.A strong linear response (r>0.999) was observed for all four aflatoxins across the tested range of 0.1 to 10.0 ng/mL.The established method provided detection and quantification limits of 0.03-0.12 μg/kg and 0.1-0.4 μg/kg, respectively.At three different concentration levels, the average recoveries were 77.3%-99.2%, and the relative standard deviations were 0.4%-5.2%.This method offers a simple, green and low-cost alternative for the rapid qualitative and quantitative analysis of 4 kinds of aflatoxins in silkworm pupae powder.

Key words： silkworm pupae powder; aflatoxins; supramolecular solvent; ultra-high performance liquid chromatography-tandem mass spectrometry

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