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桑叶中1-脱氧野尻霉素的提取、测定及其生物活性研究进展

  • 巫小慧 ,
  • 刘洁琳 ,
  • 周良付 ,
  • 张莹莹 ,
  • 曹佳程 ,
  • 路亚欣 ,
  • 唐霞 ,
  • 生庆海
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  • 1(河北农业大学 食品科技学院,河北 保定,071000)
    2(承德市农林科学院,河北 承德,067000)
    3(河北省膳食组分互作与精准营养重点实验室,河北 保定,071000)
第一作者:硕士研究生(生庆海教授和唐霞副教授为通信作者,E-mail:Sqh515517@163.com;bdzzp@163.com)

收稿日期: 2025-07-09

  修回日期: 2025-08-21

  网络出版日期: 2026-05-07

基金资助

河北省燕赵黄金台聚才计划骨干人才项目(A20240024);保定市创新能力提升专项项目(2494N015);河北农业大学引进人才科研专项(YJ2024012);承德市应用技术研究与开发暨可持续发展议程创新示范区专项基础研究项目(202404B074)

Research progress on extraction, determination, and biological activity of 1-deoxynojirimycin from mulberry leaves

  • WU Xiaohui ,
  • LIU Jielin ,
  • ZHOU Liangfu ,
  • ZHANG Yingying ,
  • CAO Jiacheng ,
  • LU Yaxin ,
  • TANG Xia ,
  • SHENG Qinghai
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  • 1(College of Food Science and Technology, Hebei Agricultural University, Baoding 071000, China)
    2(Chengde Academy of Agriculture and Forestry Sciences, Chengde 067000, China)
    3(Hebei Provincial Key Laboratory of Dietary Component Interaction and Precision Nutrition, Baoding 071000, China)

Received date: 2025-07-09

  Revised date: 2025-08-21

  Online published: 2026-05-07

摘要

1-脱氧野尻霉素(1-deoxynojirimycin,DNJ)是一种存在于桑叶中的天然生物碱,其作为有效的糖苷酶抑制剂,在降糖方面具有显著功效,并且具备降血脂、抗病毒、抗癌等多种生物活性。目前DNJ可以通过热提取、超声提取、微波提取及回流提取等多种方法进行提取。对于DNJ的测定方法主要采用高效液相色谱进行。然而,目前现有的研究对DNJ生物活性的系统性总结尚显不足。该文对DNJ的功效及其作用机制进行全面梳理,深入探讨其提取方法,测定方法以及生物活性研究的最新进展,以期为DNJ后续的研究提供一定的参考。

本文引用格式

巫小慧 , 刘洁琳 , 周良付 , 张莹莹 , 曹佳程 , 路亚欣 , 唐霞 , 生庆海 . 桑叶中1-脱氧野尻霉素的提取、测定及其生物活性研究进展[J]. 食品与发酵工业, 2026 , 52(8) : 427 -435 . DOI: 10.13995/j.cnki.11-1802/ts.043773

Abstract

1-deoxynojirimycin (DNJ) is a naturally occurring alkaloid that has been identified in mulberry leaves.It has been demonstrated that the substance under scrutiny is an effective glycosidase inhibitor, with the potential to reduce blood sugar levels.In addition to this, a number of biological activities have been observed in the substance, including the capacity to lower lipids, to act as an antiviral, and to elicit anticancer effects.The present paper sets out the various methodologies currently employed for the extraction of DNJ, including thermal extraction, ultrasonic extraction, microwave extraction and reflux extraction.The primary method for determining DNJ is high performance liquid chromatography.However, a comprehensive and methodical review of the extant literature on the biological activity of DNJ remains to be undertaken.This article comprehensively reviews the efficacy and mechanism of action of DNJ, and explores its extraction and determination methods, as well as the latest developments in research on its biological activity.The article's objective is to serve as a foundational reference for future research endeavours concerning DNJ.

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