食品与发酵工业 >

2026 , Vol. 52 >Issue 9: 20 - 28

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.043695

研究报告

格氏乳杆菌TF08-1的菌株特性及安全性评价

  • 杨埔 ,
  • 申瑾蓉 ,
  • 张丽珍
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  • (山西大学 生命科学学院,山西 太原,030006)
第一作者：博士,副教授(张丽珍教授为通信作者,E-mail:lizhen@sxu.edu.cn)

收稿日期: 2025-06-25

  修回日期: 2025-09-30

  网络出版日期: 2026-06-03

基金资助

山西省高等学校科技创新项目(2019L0104)

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Properties and safety assessment of Lactobacillus gasseri TF08-1

  • YANG Pu ,
  • SHEN Jinrong ,
  • ZHANG Lizhen
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  • (School of Life Science, Shanxi University, Taiyuan 030006, China)

Received date: 2025-06-25

  Revised date: 2025-09-30

  Online published: 2026-06-03

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摘要

格氏乳杆菌(Lactobacillus gasseri)TF08-1是分离自健康人体粪便样本的一株益生菌,具有降脂、降糖、缓解体重增长等多种益生功能。为进一步开发利用,该文研究了该菌的抑菌能力和对环境胁迫的抗逆性,并对其安全性进行评价。结果显示,格氏乳杆菌TF08-1上清液对4种致病菌具有抑菌活性。上清液中高浓度L-乳酸导致的pH下降是抑菌机制之一。进一步研究表明,该菌对消化道具有较好的耐受性,并具有较强的自聚集率和黏附能力。该菌无溶血活性,不产生生物胺,未检出抗生素抗性,遗传稳定性较强,同时对小鼠无致病性。因此,格氏乳杆菌TF08-1具有较好的益生特性、抗逆性和安全性,可以进一步开发利用。

关键词： 格氏乳杆菌; 益生菌; 自聚集; 安全性评价

本文引用格式

杨埔 , 申瑾蓉 , 张丽珍 . 格氏乳杆菌TF08-1的菌株特性及安全性评价[J]. 食品与发酵工业, 2026 , 52(9) : 20 -28 . DOI: 10.13995/j.cnki.11-1802/ts.043695

Abstract

Lactobacillus gasseri TF08-1, a probiotic candidate isolated from the fecal of a healthy female, has been reported to alleviate conditions such as hyperlipidemia, diabetes mellitus and obesity.To facilitate its further application, the antibacterial activities, stress resistances and safety profile of L. gasseri TF08-1 were evaluated.The results demonstrated antibacterial activities of the cell-free supernatant of L. gasseri TF08-1 against four pathogens with L-lactic acid as one of the antibacterial agents.L. gasseri TF08-1 also showed resistance to stimulated oral, gastric and intestinal stresses, as well as autoaggregation and surface adhesion abilities.Additionally, L. gasseri TF08-1 lacked hemolysis, did not produce biogenic amine, and was sensitive to antibiotics.It was non-pathogenic to rat and maintained phenotypic and genetic stability within 20 generations.In conclusion, L. gasseri TF08-1 showed promising probiotic properties and stress resistance, and is safe for future application.

Key words： Lactobacillus gasseri; probiotics; autoaggregation; safety assessment

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