食品与发酵工业 >

2026 , Vol. 52 >Issue 9: 109 - 117

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044155

研究报告

茶树油纳米乳液的制备及其对副溶血弧菌生物膜和群体感应的影响

  • 朱麓琳 ,
  • 杨静贤 ,
  • 植键莹 ,
  • 叶朝阳 ,
  • 蒋艳兰 ,
  • 钟青萍
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  • (广东省食品质量安全重点实验室,华南农业大学 食品学院,广东 广州,510642)
第一作者：硕士研究生(钟青萍教授为通信作者,E-mail:zhongqp@scau.edu.cn)

收稿日期: 2025-08-06

  修回日期: 2025-10-10

  网络出版日期: 2026-06-03

基金资助

广东省基础与应用基础研究基金项目(2024A1515012695);国家级大学生创新创业训练计划项目(202410564031);国家自然科学基金项目(31972046)

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Preparation of tea tree oil nanoemulsion and its effects on biofilm and quorum sensing of Vibrio parahaemolyticus

  • ZHU Lulin ,
  • YANG Jingxian ,
  • ZHI Jianying ,
  • YE Chaoyang ,
  • JIANG Yanlan ,
  • ZHONG Qingping
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  • (Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China)

Received date: 2025-08-06

  Revised date: 2025-10-10

  Online published: 2026-06-03

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摘要

该研究旨在研发一种对副溶血弧菌及其生物膜具有抑制作用的新型绿色抗菌剂。以乳液粒径、多分散系数(polymer dispersity index, PDI)、zeta电位和抑菌能力等为评价指标,以超声波时间、超声波功率及茶树油添加量为实验变量,优化茶树油纳米乳液的制备条件,并探究茶树油纳米乳液对副溶血弧菌的野生株和突变株的生物膜和群体感应的影响。结果表明,在超声波功率450 W、超声波时间20 min的条件下,所制备的茶树油纳米乳液粒径为256.3 nm,PDI值为0.184,zeta电位为67.5 mV,乳液颗粒均匀,呈水包油型,且热稳定性及贮存稳定性良好;茶树油纳米乳液可有效抑制副溶血弧菌野生株和突变株的生物膜形成和代谢活性;荧光倒置显微镜和扫描电镜观察显示,经该纳米乳液处理后副溶血弧菌的死菌数增加,且菌体形态和生物膜结构受损;该纳米乳液可降低副溶血弧菌野生株群体感应相关基因的表达水平,并可有效抑制群体感应信号分子AI-2的活性,但对突变株无显著影响,说明该纳米乳液可通过干扰副溶血弧菌的群体感应系统而达到抗生物膜作用。

关键词： 茶树油; 副溶血弧菌; 纳米乳液; 生物膜; 群体感应

本文引用格式

朱麓琳 , 杨静贤 , 植键莹 , 叶朝阳 , 蒋艳兰 , 钟青萍 . 茶树油纳米乳液的制备及其对副溶血弧菌生物膜和群体感应的影响[J]. 食品与发酵工业, 2026 , 52(9) : 109 -117 . DOI: 10.13995/j.cnki.11-1802/ts.044155

Abstract

This study aimed to develop a novel environmentally friendly antibacterial agent with inhibitory effects on Vibrio parahaemolyticus and its biofilm.To optimize the preparation conditions of tea tree oil nanoemulsion, emulsion particle size, polymer dispersity index (PDI), zeta potential, and antibacterial activity were selected as evaluation indicators, while ultrasonic time, ultrasonic power, and tea tree oil dosage served as experimental variables.Moreover, the effects of the tea tree oil nanoemulsion on the biofilm and quorum sensing (QS) of both wild-type and mutant strains of V.parahaemolyticus were investigated.The results showed that when the ultrasonic power was set at 450 W and the ultrasonic time was 20 min, the prepared tea tree oil nanoemulsion exhibited the following characteristics:a particle size of 256.3 nm, a PDI value of 0.184, and a zeta potential of 67.5 mV.The emulsion particles were uniformly distributed, and the emulsion was identified as an oil-in-water type.Furthermore, this nanoemulsion exhibited good thermal stability and storage stability.The tea tree oil nanoemulsion could effectively inhibit the biofilm formation and metabolic activity of both wild-type and mutant strains of V.parahaemolyticus.Observations under fluorescence inverted microscopy and scanning electron microscopy revealed that after treatment with this nanoemulsion, the number of dead V.parahaemolyticus cells increased, and the bacterial morphology and biofilm structure were damaged.Furthermore, the nanoemulsion reduced the expression levels of QS-related genes in the wild-type strain of V.parahaemolyticus and effectively inhibit the activity of the QS signal molecule AI-2.In contrast, no significant effects were observed on the mutant strain.These findings indicate that the nanoemulsion exerts its anti-biofilm effect by interfering with the QS system of V.parahaemolyticus.

Key words： tea tree oil; Vibrio parahaemolyticus; nanoemulsions; biofilm; quorum sensing

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