食品与发酵工业 >

2026 , Vol. 52 >Issue 9: 290 - 298

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044017

研究报告

短波紫外线照射调控玛瑙红樱桃果实贮藏品质的生理与分子响应研究

  • 聂华丽 ,
  • 吉宁 ,
  • 鲁清云 ,
  • 李昱昕 ,
  • 王瑞 ,
  • 邹伟 ,
  • 高琪
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  • 1(贵阳学院 食品科学与工程学院,贵州 贵阳,550005)
    2(贵州省生态特色食品新质化加工与贮藏全省重点实验室,贵州 贵阳,550005)
第一作者：硕士研究生(吉宁教授为通信作者,E-mail:jining552100@163.com)

收稿日期: 2025-07-23

  修回日期: 2025-10-10

  网络出版日期: 2026-06-03

基金资助

贵州省基础研究(自然科学)项目[黔科合基础MS(2025)088];贵州省生态特色食品新质化加工与贮藏全省重点实验室(黔科合平台ZSYS〔2025〕023);贵州省特色果蔬采后高质化利用科技创新人才团队建设项目[黔科合人才CXTD(2025)056];贵阳市科技人才计划项目(筑科合同[2023]48-20号);贵阳学院引进人才启动资金科研项目(GYU-KY-[2026])

收起

UV-C irradiation-regulated physiological and molecular responses of Manaohong cherry fruit quality during storage

  • NIE Huali ,
  • JI Ning ,
  • LU Qingyun ,
  • LI Yuxin ,
  • WANG Rui ,
  • ZOU Wei ,
  • GAO Qi
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  • 1(College of Food Science and Engineering, Guiyang University, Guiyang 550005, China)
    2(Guizhou Key Laboratory of New Quality Processing and Storage of Ecological Specialty Food, Guiyang 550005, China)

Received date: 2025-07-23

  Revised date: 2025-10-10

  Online published: 2026-06-03

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摘要

为探究不同剂量短波紫外线(ultraviolet C, UV-C)照射对樱桃采后贮藏生理变化的影响,该研究以玛瑙红樱桃为试材,分别使用0.5、1、2、4 kJ/m2 4种剂量的UV-C处理后,于(1.0±0.5) ℃、相对湿度(90±5)%条件下贮藏20 d。每隔5 d测定果实腐烂率与硬度,以及过氧化物酶(peroxidase, POD)、多酚氧化酶(polyphenol oxidase, PPO)、超氧化物歧化酶(superoxide dismutase, SOD)、过氧化氢酶(catalase, CAT)和抗坏血酸酶(ascorbate peroxidase, APX)的活性与基因表达水平,并采用相关性分析与主成分分析探讨各指标间的关系。结果表明,各剂量UV-C均可降低腐烂率并延缓硬度下降,其中2 kJ/m2组在贮藏末期的硬度为对照组的1.24倍,腐烂率明显低于其他处理组。该剂量能够显著提升POD、SOD、APX和CAT活性,同时抑制PPO活性,其基因表达水平亦同步上调,增强了活性氧的清除能力。相关性与主成分分析进一步证明,SOD、APX、PPO、POD和CAT活性与腐烂率及硬度呈显著相关,是决定贮藏品质的关键因素。因此,2 kJ/m2 UV-C照射可通过调控抗氧化酶系统及基因表达,有效维护玛瑙红樱桃采后品质并延长其贮藏期。

关键词： 玛瑙红樱桃; 短波紫外线照射; 采后贮藏; 抗氧化酶活性; 基因表达

本文引用格式

聂华丽 , 吉宁 , 鲁清云 , 李昱昕 , 王瑞 , 邹伟 , 高琪 . 短波紫外线照射调控玛瑙红樱桃果实贮藏品质的生理与分子响应研究[J]. 食品与发酵工业, 2026 , 52(9) : 290 -298 . DOI: 10.13995/j.cnki.11-1802/ts.044017

Abstract

To evaluate the effects of varying UV-C doses on postharvest storage physiology of Manaohong cherry, fruits were exposed to 0.5, 1, 2 or 4 kJ/m2 UV-C and stored at (1.0±0.5) ℃ and (90±5)% RH for 20 d.At 5 d intervals, decay rate and firmness were recorded, and activities and relative expression levels of peroxidase (POD), polyphenol oxidase (PPO), superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) were measured.Correlation and principal component analyses were applied to examine relationships among these traits.Findings illustrated that all UV-C treatments reduced decay and delayed firmness loss;notably, the 2 kJ/m2 treatment maintained firmness at 1.24-fold that of the control at 20 d and exhibited a significantly lower decay rate than other doses.This dose also markedly enhanced POD, SOD, APX and CAT activities while inhibiting PPO activity, with corresponding genes upregulated to boost reactive oxygen species scavenging.Correlation and principal component analyses further identified SOD, APX, PPO, POD and CAT activities as key factors determining storage quality.Accordingly, UV-C irradiation at 2 kJ/m2 offers an effective means to preserve postharvest quality and extend the storage life of Manaohong cherry by modulating its antioxidant enzyme network and gene expression.

Key words： Manaohong cherry; UV-C irradiation; postharvest storage; antioxidant enzyme activity; gene expression

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