食品与发酵工业 >

2026 , Vol. 52 >Issue 13: 68 - 76

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.045976

研究报告

发酵粘液乳杆菌S1荚膜多糖抗衰老活性研究

  • 邰孟蝶 ,
  • 赵婧 ,
  • 高永娇 ,
  • 董志昊 ,
  • 李良玉 ,
  • 王坤 ,
  • 左锋
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  • 1(黑龙江八一农垦大学 食品学院,黑龙江 大庆,163319)
    2(黑龙江八一农垦大学国家杂粮工程技术中心,黑龙江 大庆,163319)
    3(粮食副产物加工与利用教育部工程研究中心,黑龙江 大庆,163319)
第一作者:硕士研究生(王坤副教授和左锋教授为共同通信作者,E-mail:wangkun6zzu@126.com;zuofeng-518@126.com)

收稿日期: 2026-01-23

  修回日期: 2026-03-17

  网络出版日期: 2026-07-31

基金资助

黑龙江省自然科学基金优秀青年项目(YQ2021C029);黑龙江省省属本科高校“优秀青年教师基础研究支持计划”项目(YQJH2025165);黑龙江八一农垦大学“三纵”科研计划项目(ZRCPY202212)

收起

Anti-aging activity of capsular polysaccharide from Limosilactobacillus fermentum S1

  • TAI Mengdie ,
  • ZHAO Jing ,
  • GAO Yongjiao ,
  • DONG Zhihao ,
  • LI Liangyu ,
  • WANG Kun ,
  • ZUO Feng
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  • 1(College of Food science, Heilongjiang Bayi Agricultural University, Daqing 163319, China)
    2(National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, China)
    3(Engineering Research Center of Processing and Utilization of Grain By-products, Ministry of Education, Daqing 163319, China)

Received date: 2026-01-23

  Revised date: 2026-03-17

  Online published: 2026-07-31

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摘要

为探究乳酸菌荚膜多糖(capsular polysaccharides,CPS)的抗衰老活性并评估其应用于功能性食品的潜力,该研究以发酵粘液乳杆菌S1为出发菌株,采用溶菌酶法提取和DEAE-52离子交换柱层析纯化CPS,并对其结构进行表征。在此基础上,以秀丽隐杆线虫为模型,系统评价了CPS的抗衰老作用。结果表明,CPS经DEAE-52离子交换柱纯化获得一个主要组分CPS-3。该多糖的重均分子质量为4.47×104 Da,呈无规卷曲构象,由鼠李糖、葡萄糖和半乳糖组成(物质的量比1∶1.58∶12.39)。甲基化和核磁共振分析表明,CPS-3主要由β-D-Galp-(1→2,6)、β-D-Glcp-(1→2,6)、β-D-Glcp-(1→2)、β-D-Galp-(1→3)、α-L-Rhap-(1→2)、α-D-Galp-(1→3)和β-D-Galp-(1→残基构成。线虫实验结果显示,160 μg/mL CPS-3能显著提升线虫的体内抗氧化能力,并将正常培养及氧化/热应激条件下的线虫寿命分别延长19.24%、32.91%和30.02%。同时,该剂量CPS-3还可使线虫体内活性氧和脂褐素水平分别降低76.02%和59.63%,并有效改善其繁殖能力、咽泵速率与运动能力。综上,CPS-3具有明确的抗衰老活性,有望作为天然抗衰老成分应用于功能性食品。

关键词： 发酵粘液乳杆菌; 荚膜多糖; 抗氧化; 抗衰老; 秀丽隐杆线虫

本文引用格式

邰孟蝶 , 赵婧 , 高永娇 , 董志昊 , 李良玉 , 王坤 , 左锋 . 发酵粘液乳杆菌S1荚膜多糖抗衰老活性研究[J]. 食品与发酵工业, 2026 , 52(13) : 68 -76 . DOI: 10.13995/j.cnki.11-1802/ts.045976

Abstract

To investigate the anti-aging activity of capsular polysaccharides (CPS) produced by lactic acid bacteria and evaluate their application potential in functional foods, this study used Limosilactobacillus fermentum S1 as the starting strain.CPS was extracted using a lysozyme-based method, purified by DEAE-52 ion-exchange column chromatography, and subsequently characterized.On this basis, the anti-aging effects of CPS were systematically evaluated using Caenorhabditis elegans as the model organism.The results showed that purification via DEAE-52 ion-exchange chromatography yielded a major polysaccharide fraction, designated CPS-3, with an average molecular weight of 4.47×104 Da.This polysaccharide adopted a random coiled conformation and consisted of rhamnose, glucose, and galactose in a molar ratio of 1∶1.58∶12.39.Methylation and NMR analyses revealed that CPS-3 primarily consisted of β-D-Galp-(1→2,6), β-D-Glcp-(1→2,6), β-D-Glcp-(1→2), β-D-Galp-(1→3), α-L-Rhap-(1→2), α-D-Galp-(1→3), and β-D-Galp-(1→ residues.In C.elegans assays, 160 μg/mL CPS-3 significantly enhanced the in vivo antioxidant capacity of nematodes, while extending their lifespan under normal culture, oxidative stress, and thermal stress conditions by 19.24%, 32.91%, and 30.02%, respectively.Furthermore, this dosage reduced reactive oxygen species and lipofuscin levels by 76.02% and 59.63%, respectively, and improved reproductive capacity, pharyngeal pumping rate, and locomotion ability.In summary, CPS-3 exhibits clear anti-aging activity and shows promise as a natural anti-aging ingredient for functional foods.

Key words： Limosilactobacillus fermentum; capsular polysaccharide; antioxidant; anti-aging; Caenorhabditis elegans

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