食品与发酵工业 >

2026 , Vol. 52 >Issue 16: 80 - 88

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044538

研究报告

合生元对盐酸洛哌丁胺诱导便秘小鼠肠道菌群和短链脂肪酸的影响

  • 卢少帅 ,
  • 马微微 ,
  • 张紫薇 ,
  • 徐瑞鑫 ,
  • 黄莉莉
展开
  • 1(黑龙江中医药大学 药学院,黑龙江 哈尔滨,150006)
    2(长春金赛药业有限责任公司,吉林 长春,130015)
第一作者:硕士研究生(黄莉莉教授为通信作者,E-mail:908668195@qq.com)

收稿日期: 2025-09-18

  修回日期: 2026-01-06

  网络出版日期: 2026-09-10

基金资助

国家自然科学基金项目(32472302);黑龙江省自然科学基金-联合引导(LH2019H106);黑龙江省省属本科高校“优秀青年教师基础研究计划”(YQJH2023152)

收起

Modulation of gut microbiota and short-chain fatty acids by a specific synbiotic in mice with loperamide hydrochloride-induced constipation

  • LU Shaoshuai ,
  • MA Weiwei ,
  • ZHANG Ziwei ,
  • XU Ruixin ,
  • HUANG Lili
Expand
  • 1(College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150006, China)
    2(Changchun Jinsai Pharmaceutical Co.Ltd., Changchun 130015, China)

Received date: 2025-09-18

  Revised date: 2026-01-06

  Online published: 2026-09-10

Fold

摘要

基于前期研究证实该合生元具有润肠通便功能的基础,该研究旨在从微生态角度深入探究由植物乳植杆菌CCFM8661、动物双歧杆菌乳亚种XLTG11与低聚木糖(xylooligosaccharide,XOS)组成的合生元对便秘小鼠肠道菌群结构及短链脂肪酸(short chain fatty acids,SCFAs)代谢的调节作用,并分析其剂量-效应关系。根据前期功能实验确定的剂量范围,将ICR小鼠分为空白组、模型组及低、中、高剂量合生元组。灌胃14 d后建立便秘模型,收集粪便样本进行宏基因组测序和SCFAs分析。合生元干预能剂量依赖性地调节肠道菌群结构,各剂量组均提升了双歧杆菌属(Bifidobacterium)、粪杆菌属(Faecalibaculum)等有益菌属丰度,并降低了丁酸单胞菌属(Butyricimonas)等菌属丰度。α和β多样性分析显示,中剂量干预在恢复菌群多样性和群落结构方面表现出最佳趋势,而高剂量干预则引起了ACE指数与Obs指数的显著降低(P<0.05)。SCFAs分析表明,低剂量与中剂量组接近于空白组,高剂量组发生了显著变化。该合生元可通过重塑肠道菌群结构及影响SCFAs代谢缓解便秘。综合菌群结构稳态与代谢功能指标,中剂量(3×106 CFU+2 mg XOS)显示出更优的调节潜力。

关键词： 合生元; 益生菌; 便秘; 肠道菌群; 短链脂肪酸

本文引用格式

卢少帅 , 马微微 , 张紫薇 , 徐瑞鑫 , 黄莉莉 . 合生元对盐酸洛哌丁胺诱导便秘小鼠肠道菌群和短链脂肪酸的影响[J]. 食品与发酵工业, 2026 , 52(16) : 80 -88 . DOI: 10.13995/j.cnki.11-1802/ts.044538

Abstract

Previous studies confirmed the laxative effect of this synbiotic.Building on this, this study investigated its dose-dependent effects on gut microbiota and short-chain fatty acids (SCFAs) in constipated mice from a microecological perspective.A synbiotic containing Lactiplantibacillus plantarum CCFM8661, Bifidobacterium animalis subsp.lactis XLTG11, and xylooligosaccharides (XOS) was administered to ICR mice at low, medium, and high doses for 14 days.Constipation was then induced with loperamide hydrochloride.Fecal samples were analyzed via metagenomic sequencing and SCFA profiling.Synbiotic intervention dose-dependently modulated gut microbiota.All doses increased beneficial genera like Bifidobacterium and Faecalibaculum while reducing Butyricimonas.Medium-dose intervention best restored microbial diversity and structure, whereas high-dose significantly lowered ACE and observed species indices (P<0.05).SCFA profiles in low- and medium-dose groups resembled the blank group, but the high-dose group showed significant changes.This synbiotic alleviates constipation by remodeling gut microbiota and regulating SCFA metabolism.The medium dose (3×106 CFU+2 mg XOS) demonstrated optimal regulatory potential by balancing microbiota structure and metabolic function.

Key words： synbiotic; probiotics; constipation; gut microbiota; short-chain fatty acids (SCFAs)

参考文献

[1] BALLOU S, KATON J, SINGH P, et al.Chronic diarrhea and constipation are more common in depressed individuals[J].Clinical Gastroenterology and Hepatology, 2019, 17(13):2696-2703.
[2] 那日娜, 陈平.益生菌通过肠道菌群治疗功能性便秘的研究进展[J].临床医学进展, 2023, 13(5):8669-8674.
NA R N, CHEN P.Progress of probiotics in treating functional constipation through intestinal flora[J].Advances in Clinical Medicine, 2023, 13(5):8669-8674.
[3] ZHANG T, LIU W X, LU H M, et al.Lactic acid bacteria in relieving constipation:Mechanism, clinical application, challenge, and opportunity[J].Critical Reviews in Food Science and Nutrition, 2025, 65(3):551-574.
[4] SWANSON K S, GIBSON G R, HUTKINS R, et al.The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of synbiotics[J].Nature Reviews.Gastroenterology & Hepatology, 2020, 17(11):687-701.
[5] YI R K, ZHOU X, LIU T J, et al.Amelioration effect of Lactobacillus plantarum KFY02 on low-fiber diet-induced constipation in mice by regulating gut microbiota[J].Frontiers in Nutrition, 2022, 9:938869.
[6] LAI H, LI Y F, HE Y F, et al.Effects of dietary fibers or probiotics on functional constipation symptoms and roles of gut microbiota:A double-blinded randomized placebo trial[J].Gut Microbes, 2023, 15(1):2197837.
[7] WANG L L, CHAI M, WANG J L, et al.Bifidobacterium longum relieves constipation by regulating the intestinal barrier of mice[J].Food & Function, 2022, 13(9):5037-5049.
[8] 王鑫, 朱婧, 刘静, 等.低聚木糖润肠通便及调节肠道菌群功能的研究[J].食品工业科技, 2015, 36(14):359-362.
WANG X, ZHU J, LIU J, et al.Study on laxative and regulating function for the intestinal flora of xylooligosaccharides[J].Science and Technology of Food Industry, 2015, 36(14):359-362.
[9] 张紫薇, 于爽, 赵伊阳, 等.复合益生菌对便秘小鼠的润肠通便作用[J].食品科技, 2025, 50(5):36-43.
ZHANG Z W, YU S, ZHAO Y Y, et al.Laxative effect of compound probiotics on constipated mice[J].Food Science and Technology, 2025, 50(5):36-43.
[10] 王佳丽, 石磊, 李军祥, 等.基于伪无菌实验研究芪地通便方对慢传输型便秘小鼠肠道菌群的影响[J].中国中西医结合消化杂志, 2023, 31(12):920-927.
WANG J L, SHI L, LI J X, et al.Effects of Qidi Tongbian Prescription on intestinal flora in mice with slow transit constipation based on pseudo-sterile experiment[J].Chinese Journal of Integrated Traditional and Western Medicine on Digestion, 2023, 31(12):920-927.
[11] 宋晓妍, 肖灵, 刘雅萱, 等.益生菌协同黑茶调节肠道菌群缓解盐酸洛哌丁胺诱导的小鼠便秘[J].食品与发酵工业, 2025, 51 (12):294-302.
SONG X Y, XIAO L, LIU Y X, et al.Probiotics synergize with dark tea to regulate intestinal flora and alleviate loperamide-induced constipation in mice[J].Food and Fermentation Industries, 2025, 51(12):294-302.
[12] 喻喜华, 李刘若兰, 王一, 等.四种干预方式缓解便秘效果及机制研究[J].食品工业科技, 2025, 46(23):428-439.
YU X H, LI L R L, WANG Y, et al.Effects and mechanisms of four interventions in alleviating constipation[J].Science and Technology of Food Industry, 2025, 46(23):428-439.
[13] HAO M Q, SONG J, ZHAI X H, et al.Improvement of loperamide-hydrochloride-induced intestinal motility disturbance by Platycodon grandiflorum polysaccharides through effects on gut microbes and colonic serotonin[J].Frontiers in Cellular and Infection Microbiology, 2023, 13:1105272.
[14] 单艺, 王旻, 杨玉菊, 等.乳糖-N-新四糖对洛哌丁胺诱导小鼠便秘的保护作用的研究[J].食品与发酵工业, 2025, 51(10):289-296.
SHAN Y, WANG M, YANG Y J, et al.Protective effect of lacto-N-neotetraose against loperamide-induced constipation in mice[J].Food and Fermentation Industries, 2025, 51(10):289-296.
[15] MAGNE F, GOTTELAND M, GAUTHIER L, et al.The Firmicutes/Bacteroidetes ratio:A relevant marker of gut dysbiosis in obese patients？[J].Nutrients, 2020, 12(5):1474.
[16] ZHU L X, LIU W S, ALKHOURI R, et al.Structural changes in the gut microbiome of constipated patients[J].Physiological Genomics, 2014, 46(18):679-686.
[17] 中华医学会肠外肠内营养学分会, 中国人体健康科技促进会肠道微生态与肠菌移植专业委员会, 上海市预防医学会肠道微生态专业委员会.慢性便秘肠道微生态临床应用中国专家共识(2024版)[J].中华胃肠外科杂志, 2024, 27(4):326-337.
Parenteral and Enteral Nutrition Branch of the Chinese Medical Association;Chinese Society for the Promotion of Human Health Science and Technology;Committee on Gut Microecology and Fecal Microbiota Transplantation,Shanghai Preventive Medicine Association.Chinese expert consensus on the clinical diagnosis and treatment of gut microecology in chronic constipation (2024 edition)[J].Chinese Journal of Gastrointestinal Surgery, 2024, 27(4):326-337.
[18] TETT A, PASOLLI E, MASETTI G, et al.Prevotella diversity, niches and interactions with the human host[J].Nature Reviews Microbiology, 2021, 19(9):585-599.
[19] DIMIDI E, CHRISTODOULIDES S, SCOTT S M, et al.Mechanisms of action of probiotics and the gastrointestinal microbiota on gut motility and constipation[J].Advances in Nutrition, 2017, 8(3):484-494.
[20] 张晨玥, 马微微, 卢慧敏, 等.植物乳杆菌CCFM 8661调节肠道菌群及缓解便秘研究[J].食品与发酵工业, 2023, 49(18):58-65.
ZHANG C Y, MA W W, LU H M, et al.Lactobacillus plantarum CCFM 8661 to regulate intestinal microbiota and relieve constipation[J].Food and Fermentation Industries, 2023, 49(18):58-65.
[21] 黄晓丽, 王国品, 于成功.普拉梭菌与肠道疾病关系的研究进展[J].世界华人消化杂志, 2017, 25(9):810-815.
HUANG X L, WANG G P, YU C G.Advances in understanding relationship between Faecalibacterium prausnitzii and intestinal disease[J].World Chinese Journal of Digestology, 2017, 25(9):810-815.
[22] LEY R E.Prevotella in the gut:Choose carefully[J].Nature Reviews Gastroenterology & Hepatology, 2016, 13(2):69-70.
[23] 胡守奎, 徐建国.螺杆菌属及其致病性研究[J].疾病监测, 2014, 29(4):321-326.
HU S K, XU J G.Research of Helicobacter spp.and its pathogenicity[J].Disease Surveillance, 2014, 29(4):321-326.
[24] TAN J K, MACIA L, MACKAY C R.Dietary fiber and SCFAs in the regulation of mucosal immunity[J].Journal of Allergy and Clinical Immunology, 2023, 151(2):361-370.
[25] PRYDE S E, DUNCAN S H, HOLD G L, et al.The microbiology of butyrate formation in the human colon[J].FEMS Microbiology Letters, 2002, 217(2):133-139.
[26] TAKEUCHI T, MIYAUCHI E, KANAYA T, et al.Acetate differentially regulates IgA reactivity to commensal bacteria[J].Nature, 2021, 595(7868):560-564.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司