便秘是一种常见的消化系统疾病,与肠道运输和肠道屏障之间存在密切关系。副干酪乳酪杆菌(Lacticaseibacillus paracasei)已被报道对肠道运输有显著的调节作用,为了探究副干酪乳酪杆菌SMN-LBK及其复合制剂对便秘小鼠的影响,该文通过测定小鼠粪便指标、病理表征、血清胃肠调节肽、肠道菌群的变化,评估其缓解便秘症状的潜力。结果表明,副干酪乳酪杆菌SMN-LBK和复合制剂均可有效缓解小鼠的便秘症状,减轻小鼠结肠的病理损伤,调节小鼠血清中胃肠调节肽的水平。与模型组相比,复合制剂显著提高了小鼠肠道菌群的Simpson指数,重塑了肠道菌群的结构和组成,并增加乳杆菌属(Lactobacillus)、Colidextribacter、Incertae Sedis和毛螺菌科(Lachnospiraceae) NK4A136 group等有益菌丰度。此外,复合制剂富集了亚油酸代谢、苯丙氨酸、酪氨酸和色氨酸的生物合成等相关功能通路,并提高了短链脂肪酸的水平。综上,副干酪乳酪杆菌SMN-LBK及其复合制剂均能有效缓解便秘并改善小鼠的胃肠道功能,研究结果为开发缓解便秘的功能性食品提供了理论依据。
Constipation is a common digestive system disease, closely associated with intestinal transit and gut barrier function.Lacticaseibacillus paracasei is capable of regulating intestinal transit.To investigate the effects of L.paracasei SMN-LBK and its composite on constipation in mice, their potential to alleviate constipation symptoms was evaluated by assessing changes in fecal indicators, pathological characteristics, serum gastrointestinal regulatory peptides, and gut microbial alterations.The results demonstrated that both L.paracasei SMN-LBK and its composite interventions resulted in alleviation of constipation symptoms, a decrease in pathological damage in the colon, and changes in serum gastrointestinal regulatory peptides.Compared with the model group, the composite significantly increased the Simpson index of the gut microbiota, reshaped gut microbial structure and composition, and elevated the abundances of beneficial bacteria such as Lactobacillus, Colidextribacter, Incertae Sedis, and Lachnospiraceae NK4A136 group.Furthermore, the composite enriched functional pathways related to linoleic acid metabolism and the biosynthesis of phenylalanine, tyrosine, and tryptophan and increased the levels of short-chain fatty acids.In conclusion, both L.paracasei SMN-LBK and its composite significantly improved constipation and regulated gastrointestinal function in mice, providing a theoretical basis for developing functional foods targeting constipation relief.
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