食品与发酵工业 >

2026 , Vol. 52 >Issue 16: 386 - 394

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.045147

分析与检测

一种用于次氯酸盐比色-荧光双模式超快响应的微波辅助合成碳点

  • 梁海洋 ,
  • 胡晋伟 ,
  • 刘诺宇 ,
  • 郑媛 ,
  • 杨安婷 ,
  • 谭琼 ,
  • 徐炜桢
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  • 1(四川轻化工大学 食品与酿酒工程学院,四川 宜宾,644012)
    2(山西省人力资源市场,山西 太原,030000)
第一作者:硕士研究生(徐炜桢博士与谭琼硕士为共同通信作者,E-mail:xuwz0313@163.com;dore2007@163.com)

收稿日期: 2025-11-12

  修回日期: 2026-01-04

  网络出版日期: 2026-09-10

基金资助

山西省特定橡木桶发酵威士忌风味物质研究资助(HX2023177);山东省技术创新引导计划(中央引导地方科技发展资金)(YDZX2023088);四川轻化工大学科研创新团队计划(SUSE652A009)

收起

A microwave-assisted synthesis of carbon dots for hypochlorous acid colorimetric-fluorescent dual-mode ultrafast response

  • LIANG Haiyang ,
  • HU Jinwei ,
  • LIU Nuoyu ,
  • ZHENG Yuan ,
  • YANG Anting ,
  • TAN Qiong ,
  • XU Weizhen
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  • 1(School of Food and Liquor Engineering, Sichuan University of Science and Engineering, Yibin 644012, China)
    2(Shanxi Human Resources Market, Taiyuan 030000, China)

Received date: 2025-11-12

  Revised date: 2026-01-04

  Online published: 2026-09-10

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摘要

次氯酸盐(ClO-)因其强氧化性作为高效消毒剂被广泛应用在食品及饮用水领域,但其过量残留会危害人体健康,因此建立食品中快速、高效的ClO-检测方法至关重要。该研究以表没食子儿茶素没食子酸酯和盐酸多巴胺为碳源,通过3 min微波辅助快速制备出蓝绿色碳点G-CDs。当有ClO-存在情况下,其可诱导G-CDs发生聚集,导致溶液由浅黄色变为深黄色,同时引发荧光共振能量转移并导致荧光淬灭,据此构建比色-荧光双模式ClO-检测体系。实验结果表明,比色法和荧光法的检测信号均在ClO-浓度0~300 μmol/L范围内呈良好线性关系:比色法相关系数R2=0.994,检测限(limit of detection, LOD)为0.354 μmol/L;荧光法相关系数R2=0.959,检测限为0.167 μmol/L,且2种检测模式的响应时间均低于10 s。将该方法应用于实际样品水发鱿鱼检测,回收率为97.82%~107.5%,相对标准偏差(relative standard deviations, RSD)不超过2.61%。该研究建立的ClO-检测方法具有碳点合成周期短、操作简便、检测快速等优势,并且双模式检测的结果可以相互补充验证,提升检测结果的准确性,在比色-荧光双模式下用于实际食品样品中ClO-检测的可行性较高。

关键词： 次氯酸盐; 碳点; 比色法; 荧光法; 快速检测

本文引用格式

梁海洋 , 胡晋伟 , 刘诺宇 , 郑媛 , 杨安婷 , 谭琼 , 徐炜桢 . 一种用于次氯酸盐比色-荧光双模式超快响应的微波辅助合成碳点[J]. 食品与发酵工业, 2026 , 52(16) : 386 -394 . DOI: 10.13995/j.cnki.11-1802/ts.045147

Abstract

Hypochlorite (ClO-) is widely used as a highly efficient disinfectant in food processing and drinking water treatment due to its strong oxidizing ability.However, excessive residual ClO- may pose risks to human health.Therefore, it is essential to develop a rapid and efficient method for the detection of ClO- in food samples.In this study, blue-green carbon dots (G-CDs) were rapidly synthesized using epigallocatechin gallate and dopamine hydrochloride as carbon sources via a 3 min microwave-assisted approach.In the presence of ClO-, G-CDs undergo aggregation, accompanied by a color change from pale yellow to dark yellow.This aggregation also induces fluorescence resonance energy transfer, resulting in fluorescence quenching.Based on these phenomena, a colorimetric-fluorescence dual-mode sensing system for ClO- was established.The experimental results show that both colorimetric and fluorescence signals exhibit good linear relationships with ClO- concentration in the range of 0-300 μmol/L.The colorimetric method yields a correlation coefficient (R2) of 0.994 and a limit of detection (LOD) of 0.354 μmol/L, while the fluorescence method gives an R2 of 0.959 and an LOD of 0.167 μmol/L.The response time for both detection modes is less than 10 s.The proposed method was applied to the determination of ClO- in soaked squid samples, with recoveries ranging from 97.82% to 107.5% and relative standard deviations (RSD) no greater than 2.61%.This study provides a rapid and facile approach for ClO- detection with the advantages of short G-CD synthesis time, simple operation, and rapid response.The dual-mode detection strategy enables mutual verification of results, thereby improving detection accuracy.The developed system shows great potential for practical ClO- detection in food samples.

Key words： hypochlorite; carbon dots; colorimetric method; fluorescence method; fast detection

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