综述与专题评论

抗菌肽设计优化策略与异源表达研究进展

  • 乔惠敏 ,
  • 贺海桥 ,
  • 张敏 ,
  • 邓宸玺 ,
  • 廖爱美 ,
  • 潘龙 ,
  • 刘娜 ,
  • 田海龙 ,
  • 王心
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  • 1(河南工业大学 生物工程学院,河南 郑州,450001)
    2(河南工业大学 生物工程学院,河南省粮食资源转化与利用重点实验室,河南 郑州,450001)
    3(广州百仕肽生物科技有限公司,广东 广州,510450)
    4(江西生物科技职业学院 动物科学系,江西 南昌,330200)
    5(河南大学 农学院,河南 开封,475001)
第一作者:硕士研究生(王心副教授为通信作者,E-mail:wangxin@haut.edu.cn)

收稿日期: 2025-10-13

  修回日期: 2025-12-29

  网络出版日期: 2026-09-10

基金资助

河南省重点研发专项(231111310700,241111111200);河南省重大科技专项(231100110300);河南省重点研发与推广专项(科技攻关)项目(252102111068);国家重点研发计划(2023YFD1301105);江西省教育厅科学技术研究项目(GJJ2407405);江西省农牧渔业科研指导性课题;河南工业大学青年骨干教师培育计划(202410463042)

Research progress on optimization strategy of antimicrobial peptide design and heterologous expression

  • QIAO Huimin ,
  • HE Haiqiao ,
  • ZHANG Min ,
  • DENG Chenxi ,
  • LIAO Aimei ,
  • PAN Long ,
  • LIU Na ,
  • TIAN Hailong ,
  • WANG Xin
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  • 1(College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China)
    2(Henan Provincial Key Laboratory of Grain Resources Conservation and Utilization, College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China)
    3(Guangzhou Bestide Bio-Science and Technology Co.Ltd., Guangzhou 510450, China)
    4(Department of Animal Science, Jiangxi Biotech Vocational College, Nanchang 330200, China)
    5(Agricultural College, Henan University, Kaifeng 475001, China)

Received date: 2025-10-13

  Revised date: 2025-12-29

  Online published: 2026-09-10

摘要

抗生素的滥用导致耐药性问题成为全球难题,抗菌肽(antimicrobial peptides, AMPs)是理想的替代品。该文简述其传统的化学改造,以及基于数据库和AlphaFold2等工具的计算机辅助设计策略。全面综述原核、真核及植物表达系统的技术优势、挑战与应用案例。研究表明,理性分子设计与多系统协同表达可提升AMPs稳定性、活性及产量。该文指出了传统改造周期长、计算机设计依赖样本、异源表达系统存在宿主毒性、修饰差异等问题。未来应聚焦人工智能辅助设计、糖工程与跨学科融合,推动AMPs在医药、农业等领域的临床转化与规模化应用。

本文引用格式

乔惠敏 , 贺海桥 , 张敏 , 邓宸玺 , 廖爱美 , 潘龙 , 刘娜 , 田海龙 , 王心 . 抗菌肽设计优化策略与异源表达研究进展[J]. 食品与发酵工业, 2026 , 52(16) : 437 -448 . DOI: 10.13995/j.cnki.11-1802/ts.044742

Abstract

The abuse of antibiotics has led to the global challenge of antimicrobial resistance, positioning antimicrobial peptides (AMPs) as ideal alternatives. This paper briefly outlines traditional strategies for the chemical modification of AMPs, as well as computer-aided design approaches utilizing databases and tools such as AlphaFold2. It provides a comprehensive review of the technical advantages, challenges, and application cases associated with prokaryotic, eukaryotic, and plant heterologous expression systems. Studies indicate that rational molecular design combined with multi-system synergistic expression can significantly enhance the stability, biological activity, and production yield of AMPs. Furthermore, this review highlights current bottlenecks, including the time-consuming nature of traditional modifications, the sample-dependency of computer-aided design, and inherent issues in heterologous expression systems such as host toxicity and variations in post-translational modifications. Future research should prioritize artificial intelligence-assisted design, glycoengineering, and interdisciplinary integration to facilitate the clinical translation and large-scale application of AMPs in the medical and agricultural sectors.

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