食品与发酵工业 >

2026 , Vol. 52 >Issue 17: 20 - 30

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.045002

精准发酵

里氏木霉Ex-26产纤维素酶发酵特征与转录组学研究

  • 崔泽洋 ,
  • 黄倩倩 ,
  • 张彦 ,
  • 郑贤良 ,
  • 龚大春 ,
  • 喻晨 ,
  • 何俊峰 ,
  • 王中 ,
  • 余华顺 ,
  • 涂璇
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  • 1(三峡大学 生物与制药学院,湖北 宜昌,443002)
    2(安琪酵母股份有限公司,湖北 宜昌,443003)
    3(三峡大学,湖北省生物酵素工程技术研究中心,湖北 宜昌,443002)
    4(安琪酶制剂(宜昌)有限公司,湖北 宜昌,443007)
    5(农业微生物资源发掘与利用全国重点实验室,湖北 宜昌,443003)
    6(酵母功能湖北省重点实验室,湖北 宜昌,443003)
第一作者:硕士研究生(余华顺高级工程师和涂璇副教授为共同通信作者,E-mail:yuhs@angelyeast.com;87258186@qq.com)

收稿日期: 2025-10-30

  修回日期: 2026-01-25

  网络出版日期: 2026-09-21

基金资助

湖北省科技攻关计划项目(2024BCB028)

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Fermentation characteristics and transcriptomics of cellulase production by Trichoderma reesei Ex-26

  • CUI Zeyang ,
  • HUANG Qianqian ,
  • ZHANG Yan ,
  • ZHENG Xianliang ,
  • GONG Dachun ,
  • YU Chen ,
  • HE Junfeng ,
  • WANG Zhong ,
  • YU Huashun ,
  • TU Xuan
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  • 1(College of Biological & Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China)
    2(Angel Yeast Co.Ltd., Yichang 443003, China)
    3(Hubei Engineering Technology Research Center for Biological Jiaosu, China Three Gorges University, Yichang 443002, China)
    4(Angel Enzyme Preparation (Yichang) Co.Ltd., Yichang 443007, China)
    5(National Key Laboratory of Agricultural Microbiology, Yichang 443003, China)
    6(The Hubei Provincial Key Laboratory of Yeast Function, Yichang 443003, China)

Received date: 2025-10-30

  Revised date: 2026-01-25

  Online published: 2026-09-21

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摘要

里氏木霉具备高效合成与分泌完整纤维素酶系的能力。通过测定发酵周期中相关生化指标,并结合转录组测序技术,分析发酵产酶关键期72 h和产酶速率显著降低144 h基因差异表达情况。结果表明,产纤维素酶效率先增加后减缓,发酵144 h产酶效率较72 h显著降低,发酵液黏度增大,蛋白质浓度降低,氧化损伤加重。转录组分析发现存在2 773个差异表达基因,包括1 554个下调,1 219个上调,其中纤维素酶核心编码基因及关键调控因子xyr1表达显著下调。进一步分析发现,下调基因主要富集于纤维素酶合成、碳代谢及蛋白质合成等方面,上调基因多与氧化应激相关。该研究揭示了发酵后期里氏木霉Ex-26合成纤维素酶效率降低的机制,为优化发酵工艺及开发高产纤维素酶的里氏木霉菌株提供了理论支持。

关键词： 里氏木霉; 纤维素酶; 产酶效率; 转录组分析

本文引用格式

崔泽洋 , 黄倩倩 , 张彦 , 郑贤良 , 龚大春 , 喻晨 , 何俊峰 , 王中 , 余华顺 , 涂璇 . 里氏木霉Ex-26产纤维素酶发酵特征与转录组学研究[J]. 食品与发酵工业, 2026 , 52(17) : 20 -30 . DOI: 10.13995/j.cnki.11-1802/ts.045002

Abstract

Trichoderma reesei possesses a strong capacity to synthesize and secrete a complete cellulase system.Through the determination of key biochemical indicators during the fermentation cycle, coupled with transcriptome sequencing, this study analyzed the differential gene expression profiles at the critical cellulase-producing stage (72 h) and the stage characterized by a marked decline in enzyme production efficiency (144 h).Results indicated that cellulase synthesis efficiency increased initially and then decreased.The efficiency at 144 h was significantly lower than that at 72 h.Concurrently, the fermentation broth viscosity increased, protein concentration decreased, and oxidative damage became more severe.Transcriptome analysis identified 2 773 differentially expressed genes (DEGs), comprising 1 554 downregulated and 1 219 upregulated genes.The expression of cellulase-encoding genes and regulatory factor xyr1 decreased significantly.Further analysis revealed that the downregulated genes were primarily enriched in pathways related to cellulase synthesis, carbon metabolism, and protein synthesis.The upregulated genes were mostly associated with oxidative stress responses.This study elucidates the mechanism of the decreased cellulase synthesis efficiency in Trichoderma reesei Ex-26 during the later fermentation stage.The findings provide a theoretical basis for optimizing fermentation processes and developing high-yield cellulase strains.

Key words： Trichoderma reesei; cellulase; enzyme production efficiency; transcriptomic analysis

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