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DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.015833

马克斯克鲁维酵母生物转化2-苯乙醇工艺优化及耐高温特性分析

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  • 1（河南科技大学食品与生物工程学院，河南  洛阳，471023）

    2（洛阳市微生物发酵工程技术研究中心，河南  洛阳，471023）
牛明福，博士，副教授，研究方向为食品微生物学。E-mail：mingfuniu@haust.edu.cn

基金资助

国家自然科学基金(31500052)

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Optimization and characterization of 2-phenylethanol bioconversion by thermotolerant yeast Kluyveromyces marxianus

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  • 1（College of food and bioengineering, Henan University of Science and Technology, Luoyang 471023, Henan, China）

    2（Luoyang Engineering and Technology Research Center of Microbial Fermentation, Luoyang 471023, Henan, China）
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摘要

以耐高温的马克斯克鲁维酵母LY1为试验菌株，通过单因素试验和响应面法分析培养基主要成分L-苯丙氨酸、碳源、氮源等对天然香料2-苯乙醇合成的影响，优化结果为L-苯丙氨酸8.39?g/L，蔗糖43.25?g/L，酵母浸粉6.68?g/L，2-苯乙醇产量达到1.450?g/L，较优化前提高了51.0%。进一步对LY1菌株进行耐受性能和高温发酵性能分析，结果表明，36?℃时对2-苯乙醇耐受的质量浓度为2.0?g/L，42?℃高温时为1.5?g/L，合成2-苯乙醇的最佳温度范围为28?℃～32?℃，42?℃时2-苯乙醇产量为0.809?g/L，为酵母菌在高温条件下转化L-苯丙氨酸合成2-苯乙醇的研究提供参考。

关键词： 耐高温; 马克斯克鲁维酵母; 2-苯乙醇; 响应面法

本文引用格式

牛明福 , 李亚恒, 陈金帅, 等 . 马克斯克鲁维酵母生物转化2-苯乙醇工艺优化及耐高温特性分析[J]. 食品与发酵工业, 0 : 1 . DOI: 10.13995/j.cnki.11-1802/ts.015833

Abstract

The medium for the production of 2-phenylethanol (2-PE) by Kluyveromyces marxianus strain LY1 were studied with the single factor and response surface methodology. The optimized medium contains 8.39?g/L L-phenylalanine, 43.25?g/L saccharose, 6.68?g/L yeast extract. The yield of 2-PE reached up to 1.450?g/L, increased by 51.0% compared with original condition. Then strain LY1 was further analyzed for its tolerance properties and high temperatural fermentation ability. The results showed that the highest tolerance to 2-PE of strain LY1 was 2.0?g/L at 36?℃ and 1.5?g/L at 42?℃. Moreover, the yield of 2-PE reached to 0.809?g/L at 42?℃. It would provide reference for production of 2-PE by thermotolerant yeasts at high temperature.

Key words： thermotolerant; Kluyveromyces marxianus; 2-phenylethanol; response surface methodology

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