落新妇苷是一种广泛存在于植物及其加工食品中的功能性黄酮成分，我们前期研究发现落新妇苷可显著减少小鼠体重增幅、腹腔脂肪重量、及血清中甘油三酯含量。本文通过研究落新妇苷对胰脂肪酶的抑制活性，进一步探索其调节脂肪代谢的可能原因。结果表明落新妇苷对胰脂肪酶有一定的抑制作用，半抑制率浓度为105 μM，最大抑制率为56.3%；且抑制效果随二者作用时间延长而增强。酶动力学特征研究表明落新妇苷是胰脂肪酶非竞争性抑制剂，不改变其与底物的亲和程度，但使最大反应速度减小。荧光光谱滴定法表明落新妇苷会使胰脂肪酶内源荧光淬灭，并使其最大发射波长红移，二者结合常数lgK_a为5.50，结合位点数n为1.17。

Astilbin is a dihydroflavonol that is widely distributed in many foods and plants. Our previous study showed astilbin could significantly decrease the body weight gain and intraperitoneal adipose tissue weight, as well as triglyceride content in serum. In this paper, we studied the inhibitory activity of astilbin on pancreatic lipase. The results showed that astilbin had inhibitory effect on pancreatic lipase with IC50 of 105 μM and maximum inhibition rate of 56.3%. The inhibitory effect increased with the interaction time between astilbin and pancreatic lipase. Enzyme kinetics study showed that astilbin is a noncompetitive inhibitor of pancreatic lipase, which does not change its affinity with the substrate, but decrease its maximum reaction rate. The fluorescence spectra showed that astilbin could quench the endogenous fluorescence of pancreatic lipase, and make its maximum emission wavelength red shift, their binding constant (lgK_a) was 5.50 and the binding-site number was 1.17.