首先采用传统的分离方法从4 ℃有氧冷藏草鱼去皮肉片货架期终点中分离出3株优势菌株，并对其进行鉴定。随后将这3株优势菌分别回接到4 ℃有氧冷藏草鱼肉片上，判断其致腐能力。最后，采用滤纸片法测定卷丹百合（LiliumlancifoliumThunb)花叶、花蕾提取物对3株优势腐败菌的抑菌活性。结果表明，4 ℃有氧冷藏草鱼去皮肉片货架期终点优势腐败菌为腐败希瓦氏菌（Shewanella putrefaciens）、草莓假单胞菌（Pseudomonas fragi）和中间气单胞菌（Aeromonas media），其中腐败希瓦氏菌的致腐能力最强。抑菌实验表明，当加量为20μL时，卷丹百合花叶水提物对腐败希瓦氏菌的抑菌圈直径为32 mm，花蕾60%乙醇提取物对草莓假单胞菌和中间气单胞菌的抑菌圈直径分别达到10 mm和26 mm。因此，可以判断卷丹百合提取物对4℃有氧冷藏草鱼去皮肉片中货架期终点优势腐败菌具有抑菌效果。

Firstly, three dominant strains were isolated at the end of shelf life from the chilled grass carpfillets storaged at 4 ℃and the isolated pure strains were sequenced and analyzed. Subsequently, these strains were inoculated back to chilled grass carpfillets at 4 ℃ and aerobic condition. TVB-N combined with sensory evaluation were measured to evaluate the putrefying ability of these strains. Filter paper diffusion was used to determine the antibacterial activities of the floral leaf and flowerbud of the LiliumlancifoliumThunb to three kinds of dominant spoilage bacteria which have high puterfaction potential.The results demonstrated that these three isolated strains were Shewanellaputrefaciens, Aeromonas media, Pseudomonas fragirespectively, and Shewanellaputrefaciensowned the strongest putrefyingability. The floral leaf water extract of LiliumlancifoliumThunb has obxious bacteriostatic effect onShewanella putrefaciens,transparent circle diameter is 32 mm.There are some differences of the degree about beingantibacterial effect between Pseudomonas fragiand Aeromonas media from bud 60% ethanol extracts, transparent circle diameter of 10 mm and 26 mm respectively.As a result,it can be concluded that the tissue extracts from LiliumlancifoliumThunb has an antibacterial effect on the dominant spoilageat the end of shelf life from the chilled grass carp fillets storaged at 4 ℃.