亚麻籽环肽混合物抑制霉菌生长繁殖活性

展开
  • (1. 广东高校油脂生物炼制工程技术研究中心,暨南大学食品科学与工程系,广东省 广州,510632;

    2. 暨南大学—萨斯喀切温大学“油料生物炼制与营养”联合实验室,广东省 广州,510632;

    3. 加拿大萨斯喀切温大学农业与生物资源学院,加拿大 萨斯卡,S7N5A8)

刘尊,硕士,研究方向为功能油脂。Email:346803107@qq.com

基金资助

广东省自然科学基金(2015A030313335)

Antifungal activity of flaxseed cyclolinopeptides mixtures

Expand
  • 1 (Guangdong Engineering Research Center of Oil and Fat Biorefinery, Department of Food Science and Engineering, Jinan University, Guangzhou, 510632, China)

    2 (Guangdong Saskatchewan Oilseeds Joint Laboratory, Jinan University&University of Saskatchewan, Guangzhou, 510632, China)

    3 (College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon S7N5A8,Canada)

摘要

亚麻籽环肽是从亚麻籽中分离得到,生物活性尚不明确。本文研究了亚麻籽环肽混合物(Flaxseed cyclolinopeptides,FCLPs)对霉菌的抑制作用。选取宛氏拟青霉(AS 3.776)和黄曲霉(CICC 2476)作为试验菌株,于121 ℃高温灭菌前、后添加一定浓度梯度的FCLPs到马铃薯葡萄糖琼脂培养基(potato dextrose agar, PDA)中,平板点种法接种培养,测定菌落直径、孢子数和孢子萌发率。结果表明FCLPs对宛氏拟青霉孢子形成的半数抑制浓度(half-inhibition concentration, IC50)分别为0.53 mg/mL(高温灭菌)和0.34 mg/mL(未经高温灭菌);对黄曲霉孢子形成的IC50值则分别为0.31 mg/mL(高温灭菌)和0.24 mg/mL(未经高温灭菌)。高温灭菌处理后,FCLPs保持了相当的抑菌活性,对宛氏拟青霉的抑制活性最低残留76.8% (菌落生长)和76.7%(孢子形成);对于黄曲霉的抑制活性,最低可分别残留61.6%(菌落生长)和41.7%(孢子形成)。FCLPs对孢子萌发率的平均抑制率为17.8%(宛氏拟青霉)和7.8%(黄曲霉)。高效液相色谱法(high performance liquid chromatography, HPLC)分析霉菌培养后从培养基中提取的FCLPs,发现高温灭菌会降低环肽的含量,这可能是导致环肽抗菌活性下降的原因之一,但环肽中四种主要环肽的比例没有发生明显变化,且没有发现环肽霉菌代谢物产生。综上所述,FCLPs对霉菌具有一定抑制活性,对高温处理较为稳定,能耐食品的热加工,相比现有抗菌肽具有独特优势。

本文引用格式

刘 尊, 汪 勇, Martin J T Reaney, 等 .

亚麻籽环肽混合物抑制霉菌生长繁殖活性
[J]. 食品与发酵工业, 0 : 1 . DOI: 10.13995/j.cnki.11-1802/ts.014230

Abstract

Flaxseed cyclolinopeptides (FCLPs) were cyclic peptides extracted from flaxseed. Their bioactivities were not elucidated yet. To evaluate antifungal activity of these FCLPs, two strains, Paecilomyces varioti bainier (AS 3.776)and Aspergillus flavus (CICC 2476), were selected as test strains and inoculated on potato dextrose agar (PDA) with FCLPs of serial concentrations added before or after autoclave. Radial growth, spores formation and spores germination were measured. Results showed that half-inhibition concentration (IC50) on spores formation of FCLPs mixtures against Paecilomyces varioti bainier were 0.53 mg/mL (autoclaved) and 0.34 mg/mL (not autoclaved), against Aspergillus flavus were 0.31 mg/mL(autoclaved) and 0.24 mg/mL (not autoclaved) respectively. Antifungal activity of FCLPs were found mostly retained after autoclave. Residual antifungal activity against Paecilomyces varioti bainier were found as much as 76.8% (radial growth) and 76.7% ( spores formation), gainst Aspergillus flavus 61.6% (radial growth) and 41.7% ( spores formation) respectively. Results of spores germination test indicated that the average inhibition rate against Paecilomyces varioti bainier (17.8%) was higher than that against Aspergillus flavus (7.8%). After fungi culture, FCLPs were extracted from PDA and analyzed by high performance liquid chromatography (HPLC). Results indicated that autoclave decreased the content of FCLPs in the media. This may lead to the decrease of antifungal activity. In this study, FCLPs were found to have antifungal activity. And the activity partially remained after autoclave. It is indicated that FCLPs have the advantages over current antimicrobial peptides when applied in food processing involved with heat treatment

文章导航

/