毕赤酵母是一种著名的外源蛋白表达宿主。但是甲醇作为诱导剂具有毒性以及在工业上大量应用易爆炸的危险性。所以研究非甲醇诱导剂诱导毕赤酵母是研究的热点。本研究以一株表达木聚糖酶的重组毕赤酵母为试材，首先优化了甲醇诱导的培养条件，进而采用甲酸铵作为诱导剂，联合山梨醇或者甲醇诱导重组毕赤酵母表达木聚糖酶。结果表明甲醇诱导时木聚糖酶活力最高达到3 403.8U/mL，单位细胞浓度活力为97.5 U/mL OD₆₀₀。甲酸铵诱导毕赤酵母醇氧化酶1启动子的强度为甲醇诱导时的40.9%。联合0.5%甲酸铵和0.5%山梨醇时木聚糖酶活力为2 032.0 U/mL，单位细胞浓度的木聚糖酶活力为甲醇诱导时的1.54倍。这表明甲酸铵可以作为非甲醇诱导剂诱导毕赤酵母表达外源蛋白，并且联合山梨醇可以使重组毕赤酵母高效表达外源蛋白。

komagataella phaffii is a famous heterologous protein expression host. However, a major issue accompanying komagataella phaffii is methanol needed as inducer which is toxic and explosive when used at large amount of industrial scale. Therefore, non-methanol inducer is urgent needed, which is a hot topic in this research field. In this research, a recombinant komagataella phaffii expressing xylanase was used to explore non-methanol inducer. The conditions of methanol induction were optimized with result of 3403.8 U/mL xylanase, and 97.5 U/mL OD₆₀₀ specific xylanase. There was 40.9%intensity of AOX1 promoter at the condition of methanol induction was obtained at the condition of 0.5% ammounim formate.And 2032.0 U/mL xylanase was obtained at the condition of 0.5% ammonium formate and 0.5% sorbitol addition per 24 h, which was 1.54 fold of AOX1 promoter intensity at the condition of methanol induction. Therefore, ammonium formate combined with sorbitolwas an efficient inducer for heterologous protein expression of komagataella phaffii.