Abstract: A new multi-wavelength visible absorption spectroscopy method for determining residual carbamazepine in livestock and poultry meat was established. In Tris-hydrochloric acid medium of pH=3.59, fast green FCF reacted with carbamazepine formed a green ion association complex with obvious three negative absorption peaks, and a faded phenomenon appeared in visible area. The maximum negative absorption peak was located at 660 nm, and the other two negative absorption peaks located at 558 nm and 424 nm, respectively. Their linear range were within 0.05-3.8 mg/L. Within this range, Beer's law was obeyed. Their apparent molar absorptivity (κ) were 6.74×104 (660 nm), 5.71×104 (558 nm), and 5.13×104 (424 nm) L/(mol·cm), respectively, with the quantitation limits being 0.11 mg/kg (660 nm) and 0.10 mg/kg (424 nm and 558 nm), respectively. When the double wavelength (660 nm+558 nm) superposition negative absorption spectrometry was used to determine the concentration of carbamazepine, its sensitivity attained 1.25×105 L/(mol·cm) and its quantitative limit was 0.053 mg/kg. When three-wavelength (660 nm+558 nm+424 nm) superposition negative absorption spectrometry was used to determine the concentration of carbamazepine, its sensitivity attained 1.76×105 L/(mol·cm) and its quantitative limit was 0.036 mg/kg. When the most sensitive three-wavelength method was applied to determine the content of residual carbamazepine in the livestock and poultry meat with spiked recoveries, the recovery and RSD% (n=5) were found to be in the ranges of 98.4%-102% and 2.4%-3.2%, respectively.
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