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食品与发酵工业  2019, Vol. 45 Issue (1): 54-61    DOI: 10.13995/j.cnki.11-1802/ts.017766
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
培养条件对湛江等鞭金藻生长和油脂产率的影响
董学卫1,李有志1,何庆芳2,3,于金慧2,毕玉平2*
1(广西大学 生命科学与技术学院,亚热带农业生物资源保护与利用国家重点实验室,广西 南宁, 530004)
2(山东省农业科学院生物技术中心,山东省作物与畜禽品种改良生物技术重点实验室,农业部黄淮海作物遗传改良与生物技术重点开发实验室,山东 济南,250100)
3(美国阿肯色大学 应用科学系,美国)
Effects of cultivation conditions on cell growth and lipid productivity of microalgae Isochrysis zhanjiangensis
DONG Xuewei1, LI Youzhi1, HE Qingfang2,3, YU Jinhui2, BI Yuping2*
1(College of Life Science and Technology, State Key Laboratory for Conservation and Utilization of Subtropical Agro- bioresources, Guangxi University, Nanning 530004, China)
2(Biotechnology Research Center, Shandong Academy of Agricultural Science, Key Laboratory for Genetic Improvement of Crop, Animal and Poultry of Shandong Province, Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Jinan 250100, China)
3(Department of Applied Science, University of Arkansas, 72204, US)
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摘要 为提高微藻油脂产率和优化培养条件,以湛江等鞭金藻(Isochrysis zhanjiangensis)为研究对象,探讨了营养方式、光强和NaNO3浓度对微藻生长和产物积累的影响以及培养过程中氮消耗与微藻生长间的关系。结果表明,湛江等鞭金藻生长越快,对氮的吸收越多,兼养较光自养和光异养消耗更多的氮以满足生长需要。充足的氮源和兼养培养条件下,蛋白质积累较多;氮浓度和光强较低条件下,油脂积累较多。光强为100 μmol/(m2·s)、NaNO3质量浓度为75 mg/L、光异养条件下油脂含量最高为46%,生物量质量浓度为0.46 g/L;光强为100 μmol/(m2·s)、NaNO3质量浓度为750 mg/L、兼养培养时生物量质量浓度最高为2.20 g/L,油脂含量为32.77%。综合考虑油脂产率和节约成本等因素,湛江等鞭金藻最高油脂产率80.06 mg/(L·d),在光强为100 μmol/(m2·s)、NaNO3质量浓度为375 mg/L、兼养培养条件下获得,此时多不饱和脂肪酸占总脂肪酸含量也较高(30.82%),因此是生产微藻油脂的合适条件。
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董学卫
李有志
何庆芳
于金慧
毕玉平
关键词:  湛江等鞭金藻  兼养  生物量浓度  光强  油脂产率    
Abstract: In order to improve the microalgae lipid productivity and optimize its cultivation conditions, Isochrysis zhanjiangensis was used as the research object, effects of trophic modes, light intensity, and NaNO3 concentration on the growth and lipid accumulation, as well as the relationship between nitrogen consumption and growth in the course of culture were explored. The results showed that microalgae consumed more nitrogen while it grew faster. Compared to photoautotrophic and photoheterotrophic cultivation, more nitrogen was consumed to meet the growth needs under mixotrophic cultivation condition. More protein were accumulated under the conditions of sufficient nitrogen and mixotrophic cultivation, whereas more oil accumulated under the conditions of low nitrogen concentration and light intensity. Although the highest oil content of 46% was obtained under the photoheterotrophic cultivation when the light intensity was 100 μmol/(m2·s) and NaNO3 concentration was 75 mg/L, the biomass concentration was only 0.46 g/L under the same condition. The highest biomass concentration of 2.20 g/L was obtained under the mixotrophic cultivation when the light intensity was 100 μmol/(m2·s) and NaNO3 concentration was 750 mg/L. However, the oil content was only 32.77%. Considering factors, such as lipid productivity and cost-saving, the best lipid productivity of 80.06 mg/(L·d) was obtained under the mixotrophic cultivation when the light intensity was 100 μmol/(m2·s) and NaNO3 concentration was 375 mg/L. Moreover, polyunsaturated fatty acids accounted for 30.82% of the total fatty acids contents under this condition. Therefore, this is a suitable condition for I. zhanjiangensis to produce microalgae oil as a nutritional substitute.
Key words:  Isochrysis zhanjiangensis    mixotrophic cultivation    biomass concentration    light intensity    lipid productivity
收稿日期:  2018-05-14                出版日期:  2019-01-15      发布日期:  2019-02-01      期的出版日期:  2019-01-15
基金资助: 山东省农业科学院青年科研基金(2015YQN32);山东省现代农业产业技术体系建设专项资金(SDAIT-26-09);国家国际科技合作专项项目(2012DFA30450);泰山学者海外人才基金资助专项(tshw20091014)
作者简介:  博士(毕玉平研究员为通讯作者,E-mail:yupingbi@vip.sina.com)。
引用本文:    
董学卫,李有志,何庆芳,等. 培养条件对湛江等鞭金藻生长和油脂产率的影响[J]. 食品与发酵工业, 2019, 45(1): 54-61.
DONG Xuewei,LI Youzhi,HE Qingfang,et al. Effects of cultivation conditions on cell growth and lipid productivity of microalgae Isochrysis zhanjiangensis[J]. Food and Fermentation Industries, 2019, 45(1): 54-61.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.017766  或          http://sf1970.cnif.cn/CN/Y2019/V45/I1/54
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