Abstract: In order to further understand the effects of polysaccharides on the stability of emulsion stability, this study aimed to investigate the effect of konjac glucomannan (KGM) on the stability of hydrolyzed Zanthoxylum armatum DC. seed kernel glutelin (HZADSKG). The results showed that 0.3% KGM had a stable effect on the stability of HZADSKG emulsion. The emulsion containing KGM had good pH stability (5-7), ionic strength stability (0-400 mmol/L NaCl, pH=3), and thermal stability (30-90 ℃, 0 mmol/L NaCl, pH=3). This study provided references for using polysaccharides as a natural macromolecular stabilizer to enhance the stability of protein emulsion. It also provided a theoretical basis for applying HZADSKG in food industries in the future.
[1] WILLIAMS P A. Food emulsions: principles, practice, and techniques [J]. International Journal of Food Science & Technology, 2001, 36(2):223-224. [2] DICKINSON E. Hydrocolloids at interfaces and the influence on the properties of dispersed systems[J]. Food Hydrocolloids, 2003, 17(1):25-39. [3] PRINS A, BOS M A, BOERBOOM F J G, et al. Relation between surface rheology and foaming behaviour of aqueous protein solutions[J]. Studies in Interface Science, 1998, 7(98):221-265. [4] AND D G, MCCLEMENTS D J. Impact of electrostatic interactions on formation and stability of emulsions containing oil droplets coated by β-lactoglobulin-pectin complexes[J]. Journal of Agricultural & Food Chemistry, 2007, 55(2):475-485. [5] MCCLEMENTS D J. Food emulsions: principles, practices, and techniques[J]. International Journal of Food Science & Technology, 2005, 36(2):223-224. [6] JOYE I J, MCCLEMENTS D J. Emulsifying and emulsion-stabilizing properties of gluten hydrolysates[J]. Journal of Agricultural & Food Chemistry, 2014, 62(12):2 623-2 630. [7] KHOURYIEH H, PULI G, WILLIAMS K, et al. Effects of xanthan-locust bean gum mixtures on the physicochemical properties and oxidative stability of whey protein stabilised oil-in-water emulsions[J]. Food Chemistry, 2015, 167:340-348. [8] COOPER C L, DUBIN P L, KAYITMAZER A B, et al. Polyelectrolyte-protein complexes[J]. Current Opinion in Colloid & Interface Science, 2005, 10(1/2):52-78. [9] QIU Chao-ying, ZHAO Mou-ming, MCCLEMENTS D J. Improving the stability of wheat protein-stabilized emulsions: effect of pectin and xanthan gum addition[J]. Food Hydrocolloids, 2015, 43:377-387. [10] GUZEY D, MCCLEMENTS D J. Formation, stability and properties of multilayer emulsions for application in the food industry[J]. Advances in Colloid & Interface Science, 2006, 128-130:227-248. [11] JOURDAIN L, LESER M E, SCHMITT C, et al. Stability of emulsions containing sodium caseinate and dextran sulfate: Relationship to complexation in solution[J]. Food Hydrocolloids, 2008, 22(4):647-659. [12] 王旭,李昕,许朵霞,等. 大豆多糖对乳清分离蛋白-乳状液稳定性与流变特性的影响[J]. 食品工业科技, 2017, 38(19):1-6. [13] AI M C, KUROIWA T, KOBAYASHI I, et al. The influence of polysaccharide on the stability of protein stabilized oil-in-water emulsion prepared by microchannel emulsification technique[J]. Colloids & Surfaces A Physicochemical & Engineering Aspects, 2014, 440(2):136-144. [14] CHENG Jin-ju, MA Ying, LI Xi-sheng, et al. Effects of milk protein-polysaccharide interactions on the stability of ice cream mix model systems[J]. Food Hydrocolloids, 2015, 45(45):327-336. [15] 寇明钰. 花椒籽蛋白质分离提取及功能性质的研究[D]. 重庆:西南大学, 2006. [16] YUAN Hong, WANG Hui, WANG Li-xia, et al. Nutritional evaluation and functional properties of the antioxidant polypeptide from Zanthoxylum bungeanum Maxim seeds kernel protein hydrolysate[J]. Cyta Journal of Food, 2017, 15(3): 425-432. [17] 杨令叶,田呈瑞,权麻玉. 大红袍花椒籽种仁蛋白的分类研究[J]. 中国油脂, 2008, 33(9):16-18. [18] 宋燕. 花椒籽膳食纤维、蛋白质的分离提取及抗氧化肽的制备研究[D]. 雅安:四川农业大学, 2012. [19] 肖雄. 线性与非线性多糖对鸡肉肌原纤维蛋白凝胶特性的影响[D]. 合肥:合肥工业大学, 2015. [20] DASH P, GHOSH G. Proteolytic and antioxidant activity of protein fractions of seeds of Cucurbita moschata[J]. Food Bioscience, 2017, 18:1-8. [21] ELAVARASAN K, SHAMASUNDAR B A. Antioxidant and emulsion properties of freshwater carps (Catla catla, Labeo rohita, Cirrhinus mrigala) protein hydrolysates prepared using flavorzyme[J]. Food Science and Biotechnology, 2017, 26(5): 1 169-1 176. [22] TAN Yun-bing, DENG Xin-lun, LIU Tong-xun, et al. Influence of NaCl on the oil/water interfacial and emulsifying properties of walnut protein-xanthan gum[J]. Food Hydrocolloids, 2017, 72:73-80. [23] LI Yuan-yuan, KONG Bao-hua, LIU Qian, et al. Improvement of the emulsifying and oxidative stability of myofibrillar protein prepared oil-in-water emulsions by addition of zein hydrolysates[J]. Process Biochemistry, 2017, 53: 116-124. [24] LAM R S H, NICKERSON M T. Food proteins: A review on their emulsifying properties using a structure–function approach[J]. Food Chemistry, 2013, 141(2):975-984. [25] ERIC D. Hydrocolloids as emulsifiers and emulsion stabilizers[J]. Food Hydrocolloids, 2009, 23(6):1 473-1 482. [26] MCCLEMENTS D J. Food emulsions: principles, practices, and techniques[J]. International Journal of Food Science & Technology, 2004, 36(2):223-224. [27] YUAN Yi, WANG Lin, PANG Jie, et al. A Review of the development of properties and structures based on konjac glucomannan as functional materials[J]. Chinese Journal of Structural Chemistry, 2017, 36(2):346-360. [28] REN Jiao-yan, ZHAO Mou-ming, SHI J, et al. Optimization of antioxidant peptide production from grass carp sarcoplasmic protein using response surface methodology[J]. LWT - Food Science and Technology, 2008, 41(9):1 624-1 632. [29] NIU Fu-ge, ZHOU Jian-zhong, NIU De-bao, et al. Synergistic effects of ovalbumin/gum arabic complexes on the stability of emulsions exposed to environmental stress[J]. Food Hydrocolloids, 2015, 47:14-20. [30] SALMINEN H, WEISS J. Electrostatic adsorption and stability of whey protein-pectin complexes on emulsion interfaces[J]. Food Hydrocolloids, 2014, 35(1):410-419. [31] XIANG Jun, LIU Fu-guo, FAN Rui, et al. Physicochemical stability of citral emulsions stabilized by milk proteins (lactoferrin, α-lactalbumin, β-lactoglobulin) and beet pectin[J]. Colloids & Surfaces A Physicochemical & Engineering Aspects, 2015, 487:104-112.
2019, Vol. 45 
