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食品与发酵工业  2019, Vol. 45 Issue (1): 236-245    DOI: 10.13995/j.cnki.11-1802/ts.018496
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荧光免疫分析法检测食品中黄曲霉毒素的研究进展
左晓维1,雷琳1,刘河冰2*,陶晓奇1, 3*
1(西南大学 食品科学学院,重庆,400715)
2(北京维德维康生物技术有限公司,北京,100095)
3(重庆市特色食品工程技术研究中心,重庆,400715)
Research progress on detecting aflatoxins in foods using fluorescence immunoassay
ZUO Xiaowei1, LEI Lin1, LIU Hebing2*, TAO Xiaoqi1, 3*
1(College of Food Science, Southwest University, Chongqing 400715, China)
2(Beijing WDWK Biotech Co., Ltd, Beijing 100095, China)
3(Chongqing Engineering Research Center of Regional Food, Chongqing 400715, China)
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摘要 黄曲霉毒素是迄今发现的真菌毒素中毒性最强的一类,是公认的I类致癌物,在粮食、坚果、油脂、乳及其制品等多种食品中均有发现。因此,研究出准确、快速、便捷的检测方法对保障食品安全和人类健康具有重要意义。荧光免疫分析法具有灵敏度高、准确性好、检测速度快、操作简单等优势,适合现场高通量快速筛查,近年来被广泛地用于检测食品中的黄曲霉毒素。该文介绍了4类常用的荧光标记物(荧光素、量子点、上转换纳米粒子和稀土离子螯合物),并详细阐述了5种荧光免疫分析法(荧光免疫吸附法、荧光免疫层析法、荧光偏振免疫分析、荧光共振能量转移免疫分析和时间分辨免疫分析)的检测原理,以及上述方法应用于检测食品中黄曲霉毒素的国内外最新研究进展,为今后的研究提供借鉴。
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左晓维
雷琳
刘河冰
陶晓奇
关键词:  黄曲霉毒素  食品  荧光标记物  荧光免疫分析法    
Abstract: Aflatoxins are the most toxic toxins among all well-known mycotoxins. They are recognized as a Class I carcinogen, and are widely present in grains, nuts, oils, milk and their products. Therefore, developing an accurate, rapid, and efficient detection method is of great significance for ensuring food safety and for human health. Fluorescence immunoassay has advantages of high sensitivity, good accuracy, fast detection, and simple operation. Therefore, it is very suitable for on-site high-throughput rapid screening. In recent years, it has been increasingly used for detecting aflatoxins in foods. In this review, four commonly used tracers (fluorescein, quantum dots, upconverting nanoparticles, and rare earth ion chelates) were introduced. Then, principles of five fluorescence immunoassays (fluorescence-linked immunosorbent assay, fluorescence immunochromatographic assay, fluorescence polarization immunoassay, fluorescence resonance energy transfer immunoassay, and time-resolved fluoroimmunoassay) were reviewed. Besides, the latest research progress on detecting aflatoxins in foods using these methods nationally and internationally were emphasized to provide guidance for future researches.
Key words:  aflatoxin    foods    fluorescent tracer    fluorescence immunoassay
收稿日期:  2018-08-11                出版日期:  2019-01-15      发布日期:  2019-02-01      期的出版日期:  2019-01-15
基金资助: 国家自然科学基金面上项目(31672605);重庆市基础科学与前沿技术研究(cstc2017jcyjAX0313);中央高校基本科研业务费学生项目(XDJK2018D023);重庆市基础研究与前沿探索项目(cstc2018jcyjAX0242);重庆市博士后科研项目特别资助(Xm2017074);中国博士后科学基金面上资助(2016M590 855);2016 年食品安全专项计划项目(重庆市食品药品监督管理局)
作者简介:  硕士研究生(陶晓奇副教授和刘河冰博士为共同通讯作者,E-mail:77179000@qq.com,lhbaily@126.com)。
引用本文:    
左晓维,雷琳,刘河冰,等. 荧光免疫分析法检测食品中黄曲霉毒素的研究进展[J]. 食品与发酵工业, 2019, 45(1): 236-245.
ZUO Xiaowei,LEI Lin,LIU Hebing,et al. Research progress on detecting aflatoxins in foods using fluorescence immunoassay[J]. Food and Fermentation Industries, 2019, 45(1): 236-245.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.018496  或          http://sf1970.cnif.cn/CN/Y2019/V45/I1/236
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