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食品与发酵工业  2019, Vol. 45 Issue (10): 201-207    DOI: 10.13995/j.cnki.11-1802/ts.018832
  分析与检测 本期目录 | 过刊浏览 | 高级检索 |
橄榄油中甘油二酯异构体测定方法研究与应用初探
魏瑶1,2, 钟其顶1,2*, 王道兵1,2,3, 李国辉1,2
1(中国食品发酵工业研究院有限公司,北京,100015)
2(全国食品发酵标准化中心,北京,100015)
3(山东金胜粮油集团有限公司,山东 临沂,276000)
Detection method of diacylglycerols isomers in olive oil and its application
WEI Yao1,2, ZHONG Qiding1,2*, WANG Daobing1,2,3, LI Guohui1,2
1(China National Research Institute of Food and Fermentation Industries Co. LTD, Beijing 100015, China)
2(National Standardization Center of Food and Fermentation Industry, Beijing 100015, China)
3(Shandong Jinsheng Cereals and Oils Group, Linyi 276000, China)
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摘要 甘油二酯(diacylglycerols, DAGs)异构体(1,3-/1,2-DAGs)在橄榄油的品质鉴别方面具有应用价值,该异构体的比例特征可用于橄榄油新鲜程度的辨别。利用固相萃取小柱分离油脂中DAGs成分,衍生处理后经GC-FID实现了DAGs的定量测定,优化后的衍生条件为衍生温度30 ℃,衍生时间1 h。该方法最低检出限(S/N=3)为0.032 mg/mL,RSD值为1.35%~4.62%,各甘油二酯成分的回收率为73.9%~76.9%。对比分析了6种初榨橄榄油和4种精炼橄榄油,结果表明二者的DAGs异构体比例存在明显不同。该方法设备简单,操作简便,可实现对橄榄油DAGs异构体进行准确测定,为开发橄榄油的真实性检测方法和建立产品标准提供技术支持,对进口初榨橄榄油的口岸监管和市场监管均具有指导意义。
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魏瑶
钟其顶
王道兵
李国辉
关键词:  甲基硅烷化  甘油二酯  气相色谱  初榨橄榄油  真实性    
Abstract: Diacylglycerols isomers (1,3-/1,2-DAGs) play an important role in identifying olive oil quality. The ratio of DAGs isomers can indicate the freshness of olive oil. Solid phase extraction was used to extract DAGs in oil, and they were quantified by GC-FID after derivatization. Derived at 30 ℃ for 1 h was the optimal derivative condition. The lowest detection limit of this method was 0.032 mg/mL, the RSD value was 1.35%-4.62%, and the average recovery rate of each DAG was 73.9%-76.9%. It was found that the ratios of DAGs isomers in six extra-virgin olive oil and four refined olive oil were significantly different. This method was simple and easy to operate. It can be used to accurately determine DAGs isomers in olive oil. It provides technical supports for developing olive oil authenticity detection methods as well as establishing product standards. It also has guiding significance for portal and market regulations on imported extract virgin olive oil.
Key words:  methylsilanation    diacylglycerols    gas chromatography    extract virgin olive oil    authenticity
收稿日期:  2018-09-19                出版日期:  2019-05-25      发布日期:  2019-06-17      期的出版日期:  2019-05-25
基金资助: 科技部国家重点研发计划(2016YFE0113200);国家自然科学基金(31601553,31601580);科技部“十三五”重大科技计划(2016YFF0203903);欧盟地平线2020(635690-OLEUM-2020-SFS-2014-2015);痕迹科学与技术公安部重点实验室开放课题(2016FMKFKT06)
作者简介:  硕士研究生(钟其顶高级工程师为通讯作者,E-mail:zhong qiding@163.com)。
引用本文:    
魏瑶,钟其顶,王道兵,等. 橄榄油中甘油二酯异构体测定方法研究与应用初探[J]. 食品与发酵工业, 2019, 45(10): 201-207.
WEI Yao,ZHONG Qiding,WANG Daobing,et al. Detection method of diacylglycerols isomers in olive oil and its application[J]. Food and Fermentation Industries, 2019, 45(10): 201-207.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.018832  或          http://sf1970.cnif.cn/CN/Y2019/V45/I10/201
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