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食品与发酵工业  2019, Vol. 45 Issue (6): 30-35    DOI: 10.13995/j.cnki.11-1802/ts.019000
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
一株链霉菌的鉴定及其产bafilomycin A1的发酵工艺研究
周剑, 方志锴, 孙菲, 江红*
(福建省微生物研究所,福建省新药(微生物)筛选重点实验室,福州,350007)
Identification of a Streptomyces isolate and fermentation process of bafilomycin A1 production
ZHOU Jian, FANG Zhikai, SUN Fei, JIANG Hong*
(Fujian province Key Laboratory of Screening for Novel Microbial Products, Fujian Institute of Microbiology, Fuzhou 350007, China)
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摘要 提高链霉菌FIM-B0711发酵液中的抗肿瘤成分bafilomycin A1含量,通过对FIM-B0711的形态学、培养特征、生理生化分析及16S rDNA分析鉴定菌株。经鉴定FIM-B0711为灰褐色链霉菌(Streptomyces griseobrunneus)。并确定了最优的发酵培养基(g/L):可溶性淀粉40、蛋白胨30、酵母粉20、玉米浆15、CaCo3 2;最优摇瓶发酵条件:接种量5.0%,发酵周期96 h,500 mL摇瓶装量100 mL,培养温度28 ℃,摇床转速220 r/min,培养基初始pH值为7.0。 优化后发酵工艺bafilomycin A1的发酵产量较初始工艺提高了约344%。
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周剑
方志锴
孙菲
江红
关键词:  菌种鉴定  发酵  培养基优化  正交设计  bafilomycin A1    
Abstract: This study aimed to increase the level of an anti-tumor component bafilomycin A1 in Streptomyces FIM-B0711 fermentation broth. The strain FIM-B0711 was identified according to its morphology, cultural characteristics, physiochemical properties, and 16S rDNA sequence homologous analysis. The results showed that the strain FIM-B0711 was Streptomyces griseobrunneus. The optimal fermentation medium to produce bafilomycin A1 constituted 40 g/L soluble starch, 30 g/L peptone, 20 g/L yeast powder, 15 g/L corn steep liquor, and 2 g/L CaCO3. The optimal fermentation condition was as follows: 5.0% inoculum size in 100 mL medium, fermented for 96 h at 30 ℃ and at 220 r/min, with an initial pH of 7.0. The amount of bafilomycin A1 produced under optimized fermentation condition improved approximately 3.44 times than that of the original.
Key words:  strains identification    fermentation    medium optimization    orthogonal experimental design    bafilomycin A1
收稿日期:  2018-10-09                出版日期:  2019-03-25      发布日期:  2019-04-18      期的出版日期:  2019-03-25
基金资助: 国家重点研发计划资助(2018YFC0311001);十三五重大新药创制项目(2018ZX0971001-007-007)
作者简介:  硕士,副研究员(江红研究员为通讯作者,E-mail:jianghong709@163.com)。
引用本文:    
周剑,方志锴,孙菲,等. 一株链霉菌的鉴定及其产bafilomycin A1的发酵工艺研究[J]. 食品与发酵工业, 2019, 45(6): 30-35.
ZHOU Jian,FANG Zhikai,SUN Fei,et al. Identification of a Streptomyces isolate and fermentation process of bafilomycin A1 production[J]. Food and Fermentation Industries, 2019, 45(6): 30-35.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.019000  或          http://sf1970.cnif.cn/CN/Y2019/V45/I6/30
[1] WERNER G, HAGENMAIER H, ALBERT K, et al. The structure of the bafilomycins, a new group of macrolide antibiotics[J].Tetrahedron Letters,1983,24(47):5 193-5 196.
[2] 魏刚,苏超,张道敬,等.海洋放线菌Y12-26代谢产物中bafilomycins分离纯化及结构鉴定[J].中国抗生素杂志, 2011,36(8):571-575.
[3] MAUVEZIN C, NAGY P, JUHASZ G, et al. Autophagosome-lysosome fusion is independent of V-ATPase-mediated acidification[J].Nature Communications, 2015,6:7 007.
[4] LI L Q, XIE W J, PAN D, et al. Inhibition of autophagy by bafilomycin A1 promotes chemosensitivity of gastric cancer cells[J].Tumor Biology, 2016, 37(1):1-7.
[5] GERBER H P, KOEHN F E, ABRAHAM R T. The antibody-drug conjugate: An enabling modality for natural product-based cancer therapeutics [J]. Natural product reports,2013,30(5):625-639.
[6] YUAN N, SONG L, ZHANG S, et al. Bafilomycin A1 targets both autophagy and apoptosis pathways in pediatric B-cell acute lymphoblastic leukemia [J].Haematologica, 2015,100(3):345-356.
[7] CHU H Y, CHEN X, JIANG Y E, et al. Bafilomycin A1 increases the sensitivity of tongue squamous cell carcinoma cells to cisplatin by inhibiting the lysosomal uptake of platinum ions but not autophagy[J]. Cancer Letters, 2018, 423:105-112.
[8] WERNER G, HAGENMAIER H, DRAUTZ H, et al. Metabolic products of microorganisms. 224. Bafilomycins, a new group of macrolide antibiotics. Production, isolation, chemical structure and biological activity [J].The Journal of Antibiotics, 1984,37(2):110-117.
[9] YU Z, ZHAO L X, JIANG C L, et al. Bafilomycins produced by an endophytic actinomycete Streptomyces sp. YIM56209 [J]. The Journal of antibiotics,2011,64(1):159-162.
[10] CARR G, WILLIAMS D E, DIAZ-MARRERO A R, et al. Bafilomycins produced in culture by Streptomyces spp. isolated from marine habitats are potent inhibitors of autophagy [J]. Journal of Natural Products, 2010,73(3):422-427.
[11] LEE D W, NG B G, KIM B S. Increased valinomycin production in mutants of Streptomyces sp. M10 defective in bafilomycin biosynthesis and branched-chain α-keto acid dehydrogenase complex expression [J].Journal of Industrial Microbiology & Biotechnology,2015,42(11):1 507-1 517.
[12] 潘华奇,于素亚,胡江春,等.一种卡伍尔氏链霉菌及其应用:中国,CN103820351A[P].2014-05-28.
[13] 杨巍民,斯聪聪,杨星,等.海洋放线菌Y-0117农用活性代谢产物的研究[J].化学与生物工程.2013,30(1):24-27.
[14] ZHANG W, JEFFREY L F, CARLSON J C, et al. Characterization of the bafilomycin biosynthetic gene cluster from Streptomyces lohii[J].Chembiochem, 2013, 14(3):301-306.
[15] YOON H J, SUN K H, HEE K S, et al. Organization and characterization of a biosynthetic gene cluster for bafilomycin from Streptomyces griseus DSM 2608[J]. Amb Express, 2013,3(1):24.
[16] LI Z, DU L, ZHANG W, et al. Complete elucidation of the late steps of bafilomycin biosynthesis in Streptomyces lohii[J]. Journal of Biological Chemistry, 2017, 292(17):7 095-7 104.
[17] HWANG J Y, KIM S H, OH H R, et al. Analysis of a draft genome sequence of Kitasatospora cheerisanensis KCTC 2395 producing bafilomycin antibiotics[J]. Journal of Microbiology, 2015, 53(1):84-89.
[18] 张欣,杨煌建,周剑等.链霉菌BAF-0711产生的抗肿瘤活性成分的分离纯化和结构鉴定[J].天然产物研究与开发,2016,28(5):696-698;649.
[19] 阮继生,黄英.放线菌快速鉴定与系统分类[M].北京:科学出版社,2011:70.
[20] 阮继生,黄英.放线菌快速鉴定与系统分类[M].北京:科学出版社,2011:72-77.
[21] DING N,JIANG,Y,HAN L,et al.Bafilomycins and odoriferous sesquiterpenoids from Streptomyces albolongus isolated from elephas maximus feces[J].Journal of Natural Products,2016,79(4):799-805.
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