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食品与发酵工业  2019, Vol. 45 Issue (6): 16-22    DOI: 10.13995/j.cnki.11-1802/ts.019007
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
耐酸乳酸杆菌物种特异性引物设计及其在古井贡酒窖泥酒醅质量评价中的初步应用
刘倩倩1, 李俊薇2, 曹润洁2, 张会敏2, 何宏魁2*, 李安军2, 张治洲1*
1(哈尔滨工业大学(威海) 海洋科学与技术学院,山东 威海,264209)
2(安徽古井贡酒股份有限公司,安徽 毫州,236800)
Employment of PRIMER-BLAST to design species-specific primers for Lactobacillus acetotolerans and their implications in quality assessment of pitmud or Zaopei samples in Gujing Tribute liquor
LIU Qianqian1, LI Junwei2, CAO Runjie2, ZHANG Huimin2, HE Hongkui2*, LI Anjun2, ZHANG Zhizhou1*
1(Harbin Institute of Technology (Weihai), College of Marine Science and Technology, Weihai 264209, China)
2 (Anhui Gujing Tribute Co., Ltd., Bozhou 236800, China)
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摘要 古井贡酒的酿造过程中,窖泥和酒醅中发现大量耐酵乳杆菌(Lactobacillus acetotolerans),对该菌快速特异性检测非常重要。该文利用L.acetotolerans菌及大量Lactobacillus类似菌种的全基因组序列与PRIMER-BLAST方法,设计了几对L.acetotolerans特异性引物,其中2对经过系列验证具有较好的特异性,可结合QPCR技术对不同质量和不同发酵阶段的窖泥进行L.acetotolerans含量的快速检测。结果表明,古井贡酒窖泥中L.acetotolerans 含量总体明显低于酒醅,老窖泥中L.acetotolerans含量总体明显低于新窖池窖泥;而酒醅中老厂区和新厂区L.acetotolerans含量平均来看都很高,但样本之间存在明显波动。所用L.acetotolerans特异性引物可对浓香型白酒发酵样本中L.acetotolerans的相对含量测定并辅助评估窖泥酒醅质量。
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刘倩倩
李俊薇
曹润洁
张会敏
何宏魁
李安军
张治洲
关键词:  Lactobacillus acetotolerans  古井贡酒  特异性引物  窖泥  酒醅    
Abstract: When brewing Gujing Tribute liquor, a large number of Lactobacillus acetotolerans were found in both pitmud and Zaopei. Therefore, it is important to rapidly detect L. acetotolerans specifically. In this paper, whole genome sequences of Lactobacillus bacteria and PRIMER-BLAST method were used to design L. acetotolerans-specific primers. Two pairs of primers were confirmed with decent specificity and combined with quantitative polymerase chain reaction (qPCR) to rapidly measure the amount of L. acetotolerans with different qualities and at different fermentation stages in pitmud and Zaopei. The results showed that the population of L. acetotolerans in Gujing Tribute pitmud was apparently lower than that of Zaopei and was apparently lower in old pitmud than that in pitmud of new plant. Moreover, L. acetotolerans in Zaopei from both new and old plants were generally high with apparent fluctuations among samples. In general, QPCR mediated by L. acetotolerans-specific primers can be used for L. acetotolerans quantification and facilitate quality assessment of pitmud or Zaopei in strong-aroma liquor production.
Key words:  Lactobacillus acetotolerans    Gujing Tribute liquor    specific primers    pitmud    Zaopei
收稿日期:  2018-10-09                出版日期:  2019-03-25      发布日期:  2019-04-18      期的出版日期:  2019-03-25
基金资助: 国家自然科学基金(31071170);山东省自然科学基金(ZR2018MC002);GujingTribute Fund (201601)
作者简介:  硕士研究生(何宏魁高级工程师和张治洲教授为共同通讯作者,E-mail:hehongkui@gujing.com.cn,zhangzzbiox@hitwh.edu.cn)。
引用本文:    
刘倩倩,李俊薇,曹润洁,等. 耐酸乳酸杆菌物种特异性引物设计及其在古井贡酒窖泥酒醅质量评价中的初步应用[J]. 食品与发酵工业, 2019, 45(6): 16-22.
LIU Qianqian,LI Junwei,CAO Runjie,et al. Employment of PRIMER-BLAST to design species-specific primers for Lactobacillus acetotolerans and their implications in quality assessment of pitmud or Zaopei samples in Gujing Tribute liquor[J]. Food and Fermentation Industries, 2019, 45(6): 16-22.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.019007  或          http://sf1970.cnif.cn/CN/Y2019/V45/I6/16
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