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食品与发酵工业  2019, Vol. 45 Issue (10): 142-148    DOI: 10.13995/j.cnki.11-1802/ts.019590
  生产与科研经验 本期目录 | 过刊浏览 | 高级检索 |
甘肃河西走廊产区野生乳酸菌筛选及酿酒特性研究
浩楠1, 马腾臻1, 贠建民1, 杨学山1, 李爱霞1, 陈彦雄2, 韩舜愈1*
1(甘肃农业大学 食品科学与工程学院,甘肃省葡萄与葡萄酒工程学重点试验室,甘肃省葡萄与葡萄酒产业技术研发中心,甘肃 兰州,730070)
2(甘肃祁连葡萄酒业有限责任公司,甘肃 高台,734304)
Isolation and brewing characteristics of wild-type lactic acid bacteria from Gansu Hexi Corridor
HAO Nan1, MA Tengzhen1, YUN Jianmin1, YANG Xueshan1, LI Aixia1, CHEN Yanxiong2, HAN Shunyu1*
1(Gansu Agricultural University, Gansu Key Lab of Viticulture and Enology, Gansu Research and Development Center of Wine Industry, College of Food Science and Engineering, Lanzhou 730070, China)
2(Gansu Qilian Winery Co.,Ltd, Gaotai 734304,China)
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摘要 为解决葡萄酒专用乳酸菌依赖国外进口,导致葡萄酒品质同质化严重的问题,以甘肃河西走廊产区处于自然苹果酸-乳酸发酵(苹乳发酵)的葡萄酒为来源,筛选具有本土特色的优势乳酸菌。通过改良MRS培养基分离乳酸菌,经生理生化、16S rDNA基因同源性分析等鉴定,并对筛选菌株进行酿酒特性分析。获得3株具有启动苹乳发酵的小片球菌(Pediococcus parvulus,P.parvulus)且均具有较强的降酸能力,较商品乳酸菌差异不显著,其中菌株C30在低温(15 ℃)、高乙醇体积分数(14%)和高SO2质量浓度(50 mg/L)条件下的生物量显著高于商品乳酸菌。菌株C30可作为启动苹乳发酵的优势菌株,对提升河西走廊产区葡萄酒品质具有一定的应用潜力,在一定程度上推动葡萄酒品质同质化问题的解决。
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浩楠
马腾臻
贠建民
杨学山
李爱霞
陈彦雄
韩舜愈
关键词:  葡萄酒  苹乳发酵  菌株筛选  小片球菌    
Abstract: The lactic acid bacteria (LAB) for wine making in China are mostly imported from foreign manufactures, which has caused serious homogenization of wine quality. In this study, wild-type LABs from natural malolactic fermented red wine in Gansu Hexi Corridor were isolated, identified and their fermentation adaptability was examined. Three strains that could initiate malolactic fermentation and degrade acids were identified as Pediococcus parvulus. Of which, strain C30 showed significantly higher biomass yield at low fermentation temperature (15℃), high ethanol concentration (14%) and high SO2 concentration (50 mg/L) than that of commercial LAB. In conclusion, strain C30 could be used as a malolactic fermentation starter for wines and has potential value in improving the wine quality in Gansu Hexi Corridor.
Key words:  wine    malolactic fermentation    strain selection    Pediococcus parvulus
收稿日期:  2018-12-11                出版日期:  2019-05-25      发布日期:  2019-06-17      期的出版日期:  2019-05-25
基金资助: 甘肃省科技厅重点研发项目(17YF1NA060);甘肃农业大学盛彤笙科技创新基金项目(GSAU-STS-1741)
作者简介:  硕士研究生(韩舜愈教授为通讯作者,E-mail:gsndhsy@163.com)。
引用本文:    
浩楠,马腾臻,贠建民,等. 甘肃河西走廊产区野生乳酸菌筛选及酿酒特性研究[J]. 食品与发酵工业, 2019, 45(10): 142-148.
HAO Nan,MA Tengzhen,YUN Jianmin,et al. Isolation and brewing characteristics of wild-type lactic acid bacteria from Gansu Hexi Corridor[J]. Food and Fermentation Industries, 2019, 45(10): 142-148.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.019590  或          http://sf1970.cnif.cn/CN/Y2019/V45/I10/142
[1] ZAPPAROLI G, TOSI E, AZZOLINI M, et al. Bacterial inoculation strategies for the achievement of malolactic fermentation in high alcohol wines[J].South African Society for Enology & Viticulture, 2009, 30(1): 49-55.
[2] BARTOWSKY E, COSTELLO P, HENSCHKE P. Management of malolactic fermentation-wine flavor manipulation[J].Australian & New Zealand Grapegrower & Winemaker, 2002, 46(1): 10-12.
[3] BAUER R, DICKS L M T. Control of malolactic fermentation in wine. A review[J].South African Society for Enology & Viticulture, 2004,25(2): 74-88.
[4] BORDAS M, ARAQUE I, BORDONS A, et al. Differential expression of selected Oenococcus oeni genes for adaptation in wine-like media and red wine[J].Annals of Microbiology, 2015, 65(4):2 277-2 285.
[5] CINQUANTA L, DE STEFANO G, FORMATO D, et al. Effect of pH on malolactic fermentation in southern Italian wines[J]. European Food Research and Technology, 2018, 244(7):1 261-1 268.
[6] 乔慧,卢柯,杨世玲,等.中国主要葡萄酒产区酒酒球菌糖苷酶活性[J].食品科学,2014,35(23):144-150.
[7] 苏静.高抗氧化活性酒酒球菌的筛选及提升葡萄酒品质的研究[D].杨凌:西北农林科技大学,2016.
[8] MESAS J M, RODRIGUEZ M C, ALEGRE M T. Basic characterization and partial purification of β-glucosidase from cell-free extracts of Oenococcus oeni ST81[J]. Applied Microbiology, 2012, 55(3):247-255.
[9] SICO M A, BONOMO M G, SALZSNO G. Isolation and characterization of Oenococcus oeni from Aglianico wines[J].World Journal of Microbiology and Biotechnology, 2008, 24 (9): 1 829-1 835.
[10] CRUZPIO L E, POVEDA M, ALBERTO M R, et al. Exploring the biodiversity of two groups of Oenococcus oeni isolated from grape musts and wines: Are they equally diverse?[J]. Systematic & Applied Microbiology, 2017, 40(1):1-10.
[11] ROMERO J, ILABACA C, RUIZ M, et al. Oenococcus oeni in chilean red wines:Technological and genomic characterization[J]. Frontiers in Microbiology, 2018, 9:1-12.
[12] RODRÍGUEZ-NOGALES J M, VILA-CRESPO J, FERNÁ-NDEZ-FERNÁNDEZ E. Immobilization of Oenococcus oeni in lentikats to develop malolactic fermentation in wines[J]. Biotechnology Progress, 2013, 29(1):60-65.
[13] BORDAS, M, ARAQUE I, ALEGRET J O, et al. Isolation, selection, and characterization of highly ethanol-tolerant strains of Oenococcus oeni from south Catalonia[J]. International Microbiology the Official Journal of the Spanish Society for Microbiology, 2013, 16(2):113-123.
[14] JIANG J, SUMBY K M, SUNDSTROM J F, et al. Directed evolution of Oenococcus oeni strains for more efficient malolactic fermentation in a multi-stressor wine environment[J]. Food Microbiology, 2018, 73:150-159.
[15] JUEGA M, COSTANTINI A, BONELLO F, et al. Effect of malolactic fermentation by Pediococcus damnosus on the composition and sensory profile of Albariño and Caiño white wines[J]. Journal of Applied Microbiology, 2014, 116(3):586-595.
[16] 李平兰,贺稚非.食品微生物学实验原理与技术[M].北京:中国农业出版社,2014.
[17] 任晓宁,张宇,陈其玲,等.不同发酵条件对模拟葡萄酒中酒酒球菌柠檬酸代谢的影响[J].食品工业科技,2017,38(4):180-185.
[18] 钱存柔,黄仪秀.微生物学实验教程[M].北京:北京大学出版社,1999.
[19] 凌代文.乳酸细菌分类鉴定及实验方法[M].北京:中国轻工业出版社,1999.
[20] BUCHANAN R E.伯杰细菌鉴定手册.中国科学院微生物研究所,译[M].北京:科学出版社,1984.
[21] HENICKLING T, SANDINE W E, HEATHRBELL D A. Evaluation of malolactic bacteria isolated from Oregon wine[J]. Appl Environ Microbiol, 1989, 55(8): 2 010-2 016.
[22] OTIENO D O, ASTHTON J F, SHAH N P. Isoflavone phytoestrogen degradation in fermented soymilk with selected β-glucosidase producing L. acidophilus strains during storage at different temperatures[J]. International Journal of Food Microbiology, 2007, 115(1):79-88.
[23] DICKS L M T, DELLAGLIO F, COLLINS M D. Proposal to rechssify Leuconostoc oenos as Oenococcus oeni[J]. Bacteriol, 1995, 45(2): 395-397.
[24] WU Chongde, HUANG Jun, ZHOU Rongqing. Progress in engineering acid stress resistance of lactic acid bacteria[J]. Applied Microbiology and Biotechnology, 2014, 98(3):1 055-1 063.
[25] BLATTEL V, LARISIKA M, PFEIFFER P, et al. β-1,3-glucanase from Delftia tsuruhatensis strain MV01 and its potential application in vinification[J]. Applied & Environmental Microbiology, 2011, 77(3):983-990.
[26] BETTERIDGE A L, SUMBY K M, SUNDSTROM J F, et al. Application of directed evolution to develop ethanol tolerant Oenococcus oeni for more efficient malolactic fermentation[J]. Appl Microbiol Biotechnol, 2018, 102(2):921-932.
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