Determination of two alkaloids and six sweetening agents in beverages by ultraperformance liquid chromatography-tandem mass spectrometry
LIU Hong1, WANG Lin1,HU Bo2,LI Lixia1,HUANG Wei1,LIU Yun1,3*
1 (Hubei Institute for Drug Control,Wuhan 430075,China)
2 (Hubei University of Chinese Medicine,Wuhan 430065,China)
3 (College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072,China)
Abstract: A new ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with triple quadrupole method was developed in order to determine theophylline, caffeine, saccharin, acesulfame, aspartame, neotame, advantame and sucralose in beverages. Samples were ultrasonically extracted with acetonitrile and water (20∶80, v/v), followed by centrifugation and filtration. The target compound was separated by UPLC, and positive and negative electrospray ionization at multiple reaction monitoring (MRM) mode was applied. Qualitative screening was achieved with retention time and qualitative ions, and peak area and quantitative ions were used for quantification. The results showed that the limit of detection of the method was 0.003-0.5 mg/kg and the limit of quantification was 0.01-2 mg/kg. The average recovery showed a variation from 92.3% to 110.4% and the RSD was 0.9%-10.8%. Besides, a good linear relationship was found in the range of 0.001-14.088 μg/mL target compounds with correlation coefficients being above 0.995. Overall, this method was rapid, simple, sensitive and accurate, which is suitable for batch determination of the 2 alkaloids and 6 sweetening agents in beverages.
刘红,王林,胡博,等. 超高效液相色谱-串联质谱快速测定饮料中2种生物碱和6种甜味剂[J]. 食品与发酵工业, 2019, 45(17): 247-251.
LIU Hong,WANG Lin,HU Bo,et al. Determination of two alkaloids and six sweetening agents in beverages by ultraperformance liquid chromatography-tandem mass spectrometry[J]. Food and Fermentation Industries, 2019, 45(17): 247-251.