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食品与发酵工业  2019, Vol. 45 Issue (17): 227-233    DOI: 10.13995/j.cnki.11-1802/ts.020640
  分析与检测 本期目录 | 过刊浏览 | 高级检索 |
基于柑橘汁多甲氧基黄酮特征成分鉴别橙汁中宽皮桔汁
周琦1,2, 谈安群1,2, 易鑫1,2, 李贵节1,2,3*, 程玉娇1,2, 谭祥1,2, ROUSEFF RUSSELL LEE 1,2, 吴厚玖1,2, 翟雨淋1,4, 廖洪波5, 王华1,2*
1 (西南大学柑桔研究所,中国农业科学院柑桔研究所,重庆,400712)
2 (国家柑桔工程技术研究中心,重庆,400712)
3 (重庆市功能性食品协同创新中心,重庆,400067)
4 (西南大学 植物保护学院,重庆,400715)
5 (重庆市质量和标准化研究院,重庆,400023)
Identification of sweet orange juice adultery with mandarin juice based oncharacteristic polymethoxyflavones analysis
ZHOU Qi1,2,TAN Anqun1,2, YI Xin1,2, LI Guijie1,2,3*, CHENG Yujiao1,2,TAN Xiang1,2,ROUSEFF RUSSELL LEE1,2,WU Houjiu1,2, ZHAI Yulin1,4,LIAO Hongbo5,WANG Hua1,2*
1 (Citrus Research Institute, Southwest University, Chinese Academy of Agricultural Sciences, Chongqing 400712, China)
2 (National Citrus Engineering Research Center, Chongqing 400712, China)
3 (Chongqing Collaborative Innovation Center for Functional Food, Chongqing 400067, China)
4 (College of Plant Protection, Southwest University, Chongqing 400715, China)
5 (Chongqing Institute of Quality and Standardization, Chongqing 400023, China)
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摘要 榨取甜橙和宽皮柑橘各20多个品种的果汁,离心后所得上清液和果肉沉淀分别采用C18固相萃取和乙腈提取其中的多甲氧基黄酮(polymethoxyflavoned,PMFs),合并后利用高效液相色谱-二极管阵列-荧光检测串联法(high-performance liquid chromatography-diode array detection-fluorescence detection, HPLC-DAD-FLD)做定性和定量分析。以8种PMFs为变量,通过主成分分析,建立了两类果汁的鉴别模型,开发了一种经济便利的鉴别甜橙汁中是否过量掺入宽皮柑橘汁的方法。该模型对橙汁(长叶香橙汁)中掺入的宽皮柑橘汁(椪柑汁)的最低检出体积分数为10%。该方法达到国际通行标准中对橙汁中其他柑橘果汁最大添加量的检出要求,对鉴别甜橙汁纯度具有良好的效果和实际应用价值。
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周琦
谈安群
易鑫
李贵节
程玉娇
谭祥
ROUSEFF RUSSELL LEE
吴厚玖
翟雨淋
廖洪波
王华
关键词:  甜橙汁  宽皮柑橘汁  调配  多甲氧基黄酮  主成分分析    
Abstract: An economical and convenient method for identifying mandarin juice over-blended into sweet orange juice was developed. Sweet orange and mandarin juices were extracted from more than 20 varieties for each group. After centrifugation of the juices, the supernatant and fruit pulp precipitate were obtained and their polymethoxyflavones (PMFs) were separately extracted by C18 solid phase extraction and acetonitrile. The extracts were combined and PMFs were qualitatively and quantitatively analyzed by high-performance liquid chromatography-diode array detection- fluorescence detection (HPLC-DAD-FLD). The identification model to discriminate two kinds of juice was established by principal component analysis (PCA) using the 8 PMFs as variables. This model was capable of detecting a minimum 10% volume fraction of mandarin juice (using Ponkan juice as an example) added into orange juice (using Long-leaf orange juice as an example). This method meets the international standard for detecting the maximum amount of adulterated juices into orange juice and also shows good efficacy and important practical value to identify the purity of sweet orange juices.
Key words:  sweet orange juice    mandarin juice    blending    polymethoxyflavones (PMFs)    principal component analysis(PCA)
收稿日期:  2019-03-22                     发布日期:  2019-10-24      期的出版日期:  2019-09-15
基金资助: 公益性行业(农业)科研专项经费项目(201503142-12);国家柑桔工程技术研究中心开放项目(NCERC2019004);中央高校基本业务费专项(XDJK2017C014);新型果蔬汁加工关键技术及装备研发(2017YFD0400701-3)
作者简介:  硕士研究生(王华研究员和李贵节博士为共同通讯作者,E-mail:wanghua@cric.cn,liguijie@cric.cn)。
引用本文:    
周琦,谈安群,易鑫,等. 基于柑橘汁多甲氧基黄酮特征成分鉴别橙汁中宽皮桔汁[J]. 食品与发酵工业, 2019, 45(17): 227-233.
ZHOU Qi,TAN Anqun,YI Xin,et al. Identification of sweet orange juice adultery with mandarin juice based oncharacteristic polymethoxyflavones analysis[J]. Food and Fermentation Industries, 2019, 45(17): 227-233.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.020640  或          http://sf1970.cnif.cn/CN/Y2019/V45/I17/227
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