Screening of high-yield riboflavin Bacillus subtilis strain by atmospheric and room temperature plasma
GUO Jiaxin1,2,3,4, ZHANG Peiji1,2,3,4, LIU Dingyu1,2,3,4, HONG Kunqiang1,2,3,4, CHEN Tao1,2,3,4, WANG Zhiwen1,2,3,4*
1(School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China); 2(Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300350, China); 3(Frontier Science Center for Synthetic Biology, Tianjin University, Tianjin 300350, China); 4(SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350,China)
Abstract: Bacillus subtilis is an important riboflavin producing strain in microbial fermentation. It is significant importance to breed high-yield-riboflavin Bacillus subtilis strains in industrial production. In this work, the parent riboflavin-producing strain BS120 was treated with atmospheric and room temperature plasma (ARTP) for the following screening. In the first round, the production and yield of riboflavin for mutant strain BSG1 screened with 40 mg/L 8-azaguanine increased by 61.60% and 58.12%, respectively. In the second round, the production and yield of riboflavin for mutant strain BSG3 screened with 300 mg/L oligomycin increased by 83.59% and 78.76% compared with BS120, respectively. Riboflavin operon expression plasmid pMX45 was transformed into BSG3 to generate BSG5 in order to further increase the riboflavin production. The results showed that riboflavin production of BSG5 reached (4 467.08±99.47) mg/L, and the yield was (42.56±1.25) mg/g glucose, which was 140.94% and 120.52% higher than that of BS120 respectively. These mutant strains showed excellent riboflavin fermentation performance and genetic stability during the test.
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2020, Vol. 46 
