Abstract: This study analysed the fungi microbiota structure of Qula, a kind of fermented Yak milk product, using high throughput sequending technology. After extraction the metagenome DNA of Qula sample, the universal primers of fungi ITS marker gene were amplied and sequenced using Illumina high throughput sequencing technique. And the variation of microbiota structure and abundance were conducted by analyzing the Alpha diversity, species composition and Beta diversity of fungi in yak Qula samples from Gannan. The results showed that there was a high degree of fungal diversity in different Qula samples indicated in Shannon index, Simpson index, Chao1 index and ACE index. A total of 6 phyla and 123 genera of fungi were detected in the Qula samples. The common dominant phylum in all samples was Ascomycota, and the common dominant genera were Pichia and Dipodascus. The non-metric multidimensional scaling analysis and the unweighted group averaging clustering analysis were used to classify the Qula samples into three categories which were consistent with the sample sources. Adonis/PERMANOVA multivariate analysis of variance and replacement test showed that there were significant differences in fungal composition between samples from different sources (P=0.001). The Spearman correlation network diagram showed that there were positive correlation between dominant fungal communities. This study laid the foundation for the quality control of yak Qula.
马江,文鹏程,罗俏俏,等. 甘南牦牛曲拉中真菌群落结构[J]. 食品与发酵工业, 2020, 46(4): 51-56.
MA Jiang,WEN Pengcheng,LUO Qiaoqiao,et al. Study on fungi microbiota of Yak Qula in Gannan region[J]. Food and Fermentation Industries, 2020, 46(4): 51-56.
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2020, Vol. 46 
