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食品与发酵工业  2020, Vol. 46 Issue (4): 272-277    DOI: 10.13995/j.cnki.11-1802/ts.022230
  分析与检测 本期目录 | 过刊浏览 | 高级检索 |
多波长紫外吸收光谱法快速测定饮料及糕点中的山梨酸
江虹*, 庞向东, 向杰
(长江师范学院 化学化工学院,重庆, 408100)
Rapid determination of sorbic acid in drinks and pastries by multi-wavelength ultraviolet absorption spectrometry
JIANG Hong*, PANG Xiangdong, XIANG Jie
(College of Chemistry and Chemical Engineering, Yangtze Normal University, Chongqing 408100,China)
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摘要 建立快速、准确测定饮料及糕点中山梨酸的多波长紫外吸收光谱法。在pH 3.25 Tris-盐酸溶液中,碱性紫与山梨酸反应,在紫外区生成具有3 个正吸收峰的二元离子缔合物。最大吸收峰位于254 nm,另两个吸收峰分别位于305 nm 和223 nm,线性范围为0.03~1.4 mg/L,表观摩尔吸光系数(κ)分别为 4.50×104L/(mol·cm)(254 nm)、2.09×104L/(mol·cm)(305 nm)和2.53×104L/(mol·cm)(223 nm),检出限为0.028 mg/L(254 nm)、0.030 mg/L(305 nm)和0.025 mg/L(223 nm),当以单波长法(以254 nm 为例)测定时,饮料的定量限为4.66 mg/L,糕点的定量限为1.68 mg/kg。当用双波长(254 nm+305 nm)或三波长法(254 nm+305 nm+223 nm)测定时,表观摩尔吸光系数(κ)分别为6.59×104L/(mol·cm)和9.12×104L/(mol·cm),检出限分别为0.014 mg/L 和0.009 8 mg/L,饮料的定量限分别为2.33 mg/L 和1.63 mg/L,糕点的定量限分别为0.843 mg/kg 和0.590 mg/kg。以双波长法为例,样品的加标回收率和相对标准偏差RSD(n=5)分别为97.3%~102%和2.0%~2.7%。该法适于糕点及饮料中山梨酸的快速测定。
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江虹
庞向东
向杰
关键词:  山梨酸  饮料  糕点  碱性紫  多波长  紫外吸收光谱法    
Abstract: A rapid and accurate multi-wavelength ultraviolet absorption spectrometry for the determination of sorbic acid in drinks and pastries was established. In the pH 3.25 tris- hydrochloric acid solution, basic violet reacts with sorbic acid to form binary ion association complexes with three positive absorption peaks in the ultraviolet region. The maximum absorption peak was located at 254 nm, and the other two absorption peaks were located at 305 nm and 224 nm, respectively. Their linear range were 0.03~1.4 mg/L; The apparent molar absorption coefficients (κ) were 4.50×104 L/(mol·cm) (254 nm), 2.09×104 L/(mol·cm) (305 nm) and 2.53×104 L/(mol·cm) (223 nm), respectively; The detection limits were 0.028 mg/L (254 nm), 0.030 mg/L (305 nm) and 0.025 mg/L (223 nm), respectively. When measured by single-wavelength method (254 nm as an example), the quantitative limit of drinks was 4.66 mg/L and the quantitative limit of pastries was 1.68 mg/kg. When measured by dual-wavelength (254 nm+305 nm) or three-wavelength (254 nm+305 nm+223 nm) method, the apparent molar absorptivity (κ) were 6.59×104 L/(mol·cm) and 9.12×104 L/(mol·cm), respectively; The detection limits were 0.014 mg/L and 0.009 8 mg/L, respectively; The quantitative limit of beverage were 2.33 mg/L and 1.63 mg/L, respectively; The quantitative limit of pastries were 0.843 mg/kg and 0.590 mg/kg. When used dual-wavelength method as an example for measurement, the spiked recovery and relative standard deviation (RSD) (n=5) of samples were 97.3% to 102% and 2.0% to 2.7%, respectively. This method was suitable for the rapid determination of sorbic acid in pastries and drinks.
Key words:  sorbic acid    drinks    pastries    basic violet    multi-wavelength    ultraviolet absorption spectrometry
收稿日期:  2019-09-10                出版日期:  2020-02-25      发布日期:  2020-04-07      期的出版日期:  2020-02-25
基金资助: 重庆市教委科技基金资助项目(KJ1401226);长江师范学院科技基金资助项目(2018CXX117)
作者简介:  学士,教授(本文通讯作者,E-mail:jianghongch@163.com)
引用本文:    
江虹,庞向东,向杰. 多波长紫外吸收光谱法快速测定饮料及糕点中的山梨酸[J]. 食品与发酵工业, 2020, 46(4): 272-277.
JIANG Hong,PANG Xiangdong,XIANG Jie. Rapid determination of sorbic acid in drinks and pastries by multi-wavelength ultraviolet absorption spectrometry[J]. Food and Fermentation Industries, 2020, 46(4): 272-277.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.022230  或          http://sf1970.cnif.cn/CN/Y2020/V46/I4/272
[1] 刘书来,郭元帅,许凯希,等.高效液相色谱法测定秘鲁鱿鱼肌肉中的有机酸[J].食品与发酵工业,2015,41(10):135-139.
[2] IRINA T, DARIA K, KIRA S, et al. A simple and highly-available microextraction of benzoic and sorbic acids in beverages and soy sauce samples for high performance liquid chromatography with ultraviolet detection [J]. Journal of Chromatography A, 2019,1 588:1-7.
[3] MARION P C, BEATRIZ DA S F, BRUNO R C C L, et al. Simultaneous analysis of carbohydrates and organic acids by HPLC-DAD-RI for monitoring goat's milk yogurts fermentation [J]. Talanta,2016,152(1):162-170.
[4] ZHENG Huiwen, ZHANG Qiuyun, QUAN Junping, et al. Determination of sugars, organic acids, aroma components, and carotenoids in grapefruit pulps [J]. Food Chemistry,2016,205:112-121.
[5] 于淼,阮成江,李贺,等.HPLC法测定10 个引自北美蓝莓品种果实中的糖酸组分及含量[J].食品工业科技,2016,37(10):77-81.
[6] 张霞,刘志华,杨光宇,等.固相萃取富集/气相色谱法测定烟草中的9 种有机酸[J].分析测试学报,2014,33(5):545-550.
[7] TAHER A K, KHALIL F, ARAM A K. Optimized dispersive liquid-liquid microextraction and determination of sorbic acid and benzoic acid in beverage samples by gas chromatography [J]. Food Analytical Methods,2012,5 (3):351-358.
[8] SUN J, TAN L, CAO L, et al. Determination of 25 preservatives in cosmetics by gas chromatography-tandem mass spectrometry [J]. Chinese journal of chromatography, 2019,37(3):331-339.
[9] 王李平,汤逊尤,张志军,等.响应面优化-气相色谱质谱联用测定食品中山梨酸、苯甲酸和肉桂酸[J].分析试验室,2018,37(9):1 020-1 024.
[10] DING M Z, LIU W X, PENG J, et al. Simultaneous determination of seven preservatives in food by dispersive liquid-liquid microextraction coupled with gas chromatography-mass spectrometry [J]. Food Chemistry,2018,269:187-192.
[11] SAGANDYKOVA G N, ALIMZHANOVA M B, NURZHANOVA Y T, et al. Determination of semi-volatile additives in wines using SPME and GC-MS [J]. Food Chemistry,2017,220:162-167.
[12] 李艳美,李芳,粟有志,等.QuEChERS前处理结合高效液相色谱-串联质谱法同时测定酵母产品中4 种抗氧化剂和5 种防腐剂[J].食品与发酵工业,2019,45(12):215-221.
[13] 张勋,刘韬,吴连鹏,等.高效液相色谱-串联质谱法测定多种食品中7 种酚类抗氧化剂和对羟基苯甲酸酯类防腐剂[J].食品与发酵工业,2016,42(11):206-211.
[14] 马帅,王纪华,高媛,等.超高效液相色谱-串联质谱法同时测定5 个产地花椰菜和西兰花中的23 种酚酸类化合物[J].食品科学,2018,39(4):176-187.
[15] LUCIANO M, HEITOR D, LEANDRO A S P, et al. A multi-purpose tool for food inspection: Simultaneous determination of various classes of preservatives and biogenic amines in meat and fish products by LC-MS [J]. Talanta, 2018,178:1 053-1 066.
[16] HO J K, MI J L, HYE J P, et al. Simultaneous determination of synthetic food additives in kimchi by liquid chromatography-electrospray tandem mass spectrometry [J]. Food Science and Biotechnology, 2018,27(3):877-882.
[17] 黄思棋,刘晓雪,张华,等.LC-MS法测定果蔬中6种有机酸[J].食品科技,2016,41(1):275-279.
[18] JIRI Z, ZDENKA F, MIODRAG M, et al. Various instrumental approaches for determination of organic acids in wines [J]. Food Chemistry,2016,194(8):432-440.
[19] AUNG H P, PYELL U. In-capillary derivatization with o-phthalaldehyde in the presence of 3-mercaptopropionic acid for the simultaneous determination of monosodium glutamate, benzoic acid, and sorbic acid in food samples via capillary electrophoresis with ultraviolet detection [J]. Journal of Chromatography A, 2016, 1449:156-165.
[20] TOMOHARU F, TAKAHITO I, KOJI K, et al. Direct quantitation of the preservatives benzoic and sorbic acid in processed foods using derivative spectrophotometry combined with micro dialysis [J]. Food Chemistry, 2018,240:386-390.
[21] ABDOLHOSSEIN N, FARNAZ F, SAHELEH S, et al. Coupling of air-assisted liquid–liquid microextraction method with partial least squares for simultaneous spectrophotometric determination of some preservatives [J]. Journal of the Iranian Chemical Society, 2017,14(3):643-653.
[22] FRANCIELLY F S D J, ALINE G C, LAIS D A P, et al. Simple and fast method for simultaneous determination of propionate and sorbate in bread by capillary electrophoresis with UV spectrophotometric detection [J]. Journal of Food Composition and Analysis, 2018,72:93-96.
[23] 闫家伟.紫外分光光度法测定饮料中山梨酸钾的含量[J].南阳师范学院学报,2007,6(6):47-48.
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