Abstract: Due to the complexity of factors affecting starch retrogradation and the limitations of research methods, the mechanism of retrogradation symbiosis based on the short-term retrogradation stage dominated by amylose and the long-term retrogradation stage dominated by amylopectin has not been systematically elaborated. Based on this, short-term retrograded amylose crystal seeds were prepared in this study; FTIR and 13C CP/MAS NMR were used to study the process and regularity of long-term retrogradation induced by them, and the correlation between short-term and long-term retrogradation was revealed. The results showed that the granularity of amylose crystal seeds ranged from 200 to 450 nm. After the addition of amylose crystal seeds, the short-range orderliness of retrograded starch increased from 0.670 to a maximum of 0.887, and double-helix content increased from 14.86% to a maximum of 22.80%, respectively. The significant increase in short-range orderliness and double-helix content of retrograded starch indicated that the prepared amylose crystal seeds promoted the long-term retrogradation process prominently. The synergistic growth effect of crystals enriches the common mechanism of starch retrogradation.
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