Extraction of antimicrobial peptide brevilaterin fromBrevibacillus laterosporus by macroporous resin
NING Yawei1, ZHANG Mingxing1, SU Dan1, FU Yunan1, HOU Linlin1, WANG Zhixin1, JIA Yingmin2*
1(College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China) 2(School of Food and Health, Beijing Technology and Business University, Beijing 100048, China)
Abstract: The antimicrobial peptide brevilaterin from Brevibacillus laterosporus has the advantages of high safety, broad antibacterial spectrum and good thermal stability. However, the extraction process of brevilaterin was tedious, which resulted in the high production cost and difficulty in large scale production. Hence, here we aimed to develop an efficient extraction technology of brevialterin. First, the macroporous resins XAD-7HP was screened from eight resins by evaluating the adsorption-desorption ability, and then the adsorption-desorption condition was optimized. The recovery rate reached 82% under the optimal conditions: addition of 30 g/L XAD-7HP, adsorption at 35 ℃ for 1.5 h, then adding 70% ethanol and desorption for 2 h. Then, the adsorption mechanism was investigated by adsorption isotherms and thermodynamics. The results showed that the adsorption of brevilaterin by XAD-7HP fitted Langmuir model and pseudo-second-order kinetics, suggesting that it belongs to chemical adsorption of monolayer. Besides, the results on Elovich models demonstrated that the adsorption process was mainly divided into three stages: boundary layer diffusion, particle diffusion and final equilibrium. In conclusion, the extraction process of brevilaterin by macroporous resin was green and environmentally friendly, easy to operation, and cost-saving, which provids technical support for the industrial production of brevilaterin.
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2020, Vol. 46 
