Extraction of antimicrobial peptide brevilaterin fromBrevibacillus laterosporus by macroporous resin
NING Yawei1, ZHANG Mingxing1, SU Dan1, FU Yunan1, HOU Linlin1, WANG Zhixin1, JIA Yingmin2*
1(College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China) 2(School of Food and Health, Beijing Technology and Business University, Beijing 100048, China)
Abstract: The antimicrobial peptide brevilaterin from Brevibacillus laterosporus has the advantages of high safety, broad antibacterial spectrum and good thermal stability. However, the extraction process of brevilaterin was tedious, which resulted in the high production cost and difficulty in large scale production. Hence, here we aimed to develop an efficient extraction technology of brevialterin. First, the macroporous resins XAD-7HP was screened from eight resins by evaluating the adsorption-desorption ability, and then the adsorption-desorption condition was optimized. The recovery rate reached 82% under the optimal conditions: addition of 30 g/L XAD-7HP, adsorption at 35 ℃ for 1.5 h, then adding 70% ethanol and desorption for 2 h. Then, the adsorption mechanism was investigated by adsorption isotherms and thermodynamics. The results showed that the adsorption of brevilaterin by XAD-7HP fitted Langmuir model and pseudo-second-order kinetics, suggesting that it belongs to chemical adsorption of monolayer. Besides, the results on Elovich models demonstrated that the adsorption process was mainly divided into three stages: boundary layer diffusion, particle diffusion and final equilibrium. In conclusion, the extraction process of brevilaterin by macroporous resin was green and environmentally friendly, easy to operation, and cost-saving, which provids technical support for the industrial production of brevilaterin.
 李冠楠, 夏雪娟, 隆耀航, 等. 抗菌肽的研究进展及其应用[J]. 动物营养学报, 2014, 26(1): 17-25.  剧建格, 于宏伟, 韩军, 等. 广谱高效抑菌物质产生菌的筛选及鉴定[J]. 微生物学通报, 2009, 36(5): 689-693.  马俊美, 宁亚维, 王志新, 等. 侧孢短芽孢杆菌抗菌肽的结构与性质[J]. 食品与生物技术学报, 2016, 35(6): 629-634.  LV Cheng, YANG Jie, LIU Rui, et al. A comparative study on the adsorption and desorption characteristics of flavonoids from honey by six resins[J]. Food Chemistry, 2018, 268: 424-430.  LIU Yaxian, HUA Xiao, WANG Mingming, et al. Purification of the mother liquor sugar from industrial stevia production through one-step adsorption by non-polar macroporous resin[J]. Food Chemistry, 2019, 274: 337-344.  LIU Changqing, JIAO Ruihua, YAO Lingyun, et al. Adsorption characteristics and preparative separation of chaetominine from Aspergillus fumigatus mycelia by macroporous resin[J]. Journal of Chromatography B, 2016, 1 015-1 016: 135-141.  YANG Huirong, ZONG Xuyan, Cui Chun, et al. Wheat gluten hydrolysates separated by macroporous resins enhance the stress tolerance in brewer’s yeast[J]. Food Chemistry, 2018, 268: 162-170.  王广贤. 侧孢短芽孢杆菌产抗菌肽发酵条件的优化以及抑菌性质的初步研究[D]. 石家庄: 河北科技大学, 2011.  HECHARD Y, DERIJARD B, LETELLIER F, et al. Characterization and purification of mesentericin Y105, an anti-Listeria bacteriocin from Leuconostoc mesenteroides [J]. Journal of General Microbiology, 1992, 138(12): 2 725-2 731.  AKSAKAL O, UCUN H. Equilibrium, kinetic and thermodynamic studies of the biosorption of textile dye (Reactive Red 195) onto Pinus sylvestris L.[J]. Journal of Hazardous Materials, 2010, 181(1-3): 666-672.  KIM M R, KIM W C, LEE D Y, et al. Recovery of narirutin by adsorption on a non-ionic polar resin from a water-extract of Citrus unshiu peels[J]. Journal of Food Engineering, 2007, 78(1):27-32.  JIA Guantao, LU Xiuyang. Enrichment and purification of madecassoside and asiaticoside from Centella asiatica extracts with macroporous resins[J]. Journal of Chromatography A, 2008, 1 193(1-2): 136-141.  XU Zhaoyi, ZHANG Quanxing, CHEN Jinlong, et al. Adsorption of naphthalene derivatives on hypercrosslinked polymeric adsorbents[J]. Chemosphere, 1999, 38(9): 2 003-2 011.  CHANDRASEKHAR J, MADHUSUDHAN M C, RAGHAVARAO K S M S. Extraction of anthocyanins from red cabbage and purification using adsorption[J]. Food and Bioproducts Processing, 2012, 90(4): 615-623.  LI Bing, WANG Chunlan, CHEN Xiaomei, et al. Highly specific separation for antitumor Spiropreussione A from endophytic fungal [Preussia sp.] fermentation broth by one-step macroporous resins AB-8 treatment[J]. Journal of Chromatography B, 2013, 938: 1-7.  BRUNA F, CELIS R, PAVLOVIC I, et al. Layered double hydroxides as adsorbents and carriers of the herbicide (4-chloro-2-methylphenoxy) acetic acid (MCPA): Systems Mg-Al, Mg-Fe and Mg-Al-Fe[J]. Journal of Hazardous Materials, 2009, 168(2-3): 1 476-1 481.  ZHENG Hong, WANG Yang, ZHENG Yan, et al. Equilibrium, kinetic and thermodynamic studies on the sorption of 4-hydroxyphenol on Cr-bentonite[J]. Chemical Engineering Journal, 2008, 143(1-3): 117-123  SALTALI K, SARI A, AYDIN M. Removal of ammonium ion from aqueous solution by natural Turkish (YIldIzeli) zeolite for environmental quality[J]. Journal of Hazardous Materials, 2007, 141(1): 258-263.  HO Y S, HUANG C T, HUANG H E. Equilibrium sorption isotherm for metal ions on tree fern[J]. Process Biochemistry, 2002, 37(12):1 421-1 430.  ZHENG Hong, LIU Donghong, ZHENG Yan, et al. Sorption isotherm and kinetic modeling of aniline on Cr-bentonite[J]. Journal of Hazardous Materials, 2009, 167(1-3): 141-147.  GARCÍA-ZUBIRI I X, GONZÁLEZ-GAITANO G, ISASI J R. Sorption models in cyclodextrin polymers: Langmuir, Freundlich, and a dual-mode approach[J]. Journal of Colloid and Interface Science, 2009, 337(1): 11-18.  AN Fuqiang, WU Ruiyan, LI Min, et al. Adsorption of heavy metal ions by iminodiacetic acid functionalized D301 resin: Kinetics, isotherms and thermodynamics[J]. Reactive and Functional Polymers, 2017, 118: 42-50.  LIU Yongfeng, DI Duolong, BAI Qingqing, et al. Preparative separation and purification of rebaudioside a from steviol glycosides using mixed-mode macroporous adsorption resins[J]. Journal of Agricultural and Food Chemistry, 2011, 59(17): 9 629-9 636.  CHANG Xiulian, WANG Dong, CHEN Biyun, et al. Adsorption and desorption properties of macroporous resins for anthocyanins from the calyx extract of roselle (Hibiscus sabdariffa L.)[J]. Journal of Agricultural and Food Chemistry, 2012, 60(9): 2 368-2 376.  YANG Qingyun, ZHAO Mouming, LIN Lianzhu. Adsorption and desorption characteristics of adlay bran free phenolics on macroporous resins[J]. Food Chemistry, 2016, 194: 900-907.  DONG Yi, ZHAO Mouming, SUN-WATERHOUSE D, et al. Absorption and desorption behaviour of the flavonoids from Glycyrrhiza glabra L. leaf on macroporous adsorption resins[J]. Food Chemistry, 2015, 168: 538-545.  CHIEN S H, CLAYTON W R. Application of elovich equation to the kinetics of phosphate release and sorption in soils[J]. Soil Science Society of America Journal, 1980, 44(2): 265-268.  HO Y S, MCKAY G. The kinetics of sorption of divalent metal ions onto sphagnum moss peat[J]. Water Research, 2000, 34(3): 735-742.  LORENC-GREBOWSKE E, GRYGLEWICZ G. Adsorption of lignite-derived humic acids on coal-based mesoporous activated carbons[J]. Journal of Colloid and Interface Science, 2005, 284(2): 416-423.