Screening and identification of a cellulase-producing bacterium from distiller′s grains and the enzymatic property
SONG Lili1*, WEN Ge1, HUO Shanhao1, HU Xiaolong1,2, YANG Xu1, ZHANG Zhiping1
1(School of Food and Bio-engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China); 2(Postdoctoral Research & Development Base, Yangshao Distillery Co. Ltd., Sanmenxia 472000, China)
Abstract: In order to obtain cellulase-producing bacteria from distiller′s grains, a byproduct of Chinese Baijiu, Congo red agar and shaking-flask fermentation method were used for screening. The isolate was identified by morphological and molecular biology methods combined with analysis of physicochemical characteristics, plus with the enzymatic characteristics studied. The results showed that a total of 16 cellulase-producing strains were screened, among which the strain YS10-2 possessed the highest enzyme activity of 36.73 U/mL on carboxy methyl cellulose (CMC), and was identified as Bacillus megaterium. The optimum reaction temperature and pH of the extracellular cellulase were 50 ℃ and 5.0, respectively. The cellulase was stable to both heat and weak acid. The enzyme activity was promoted by Ca2+, Zn2+, Mg2+, and inhibited by Cu2+ and Ba2+. The optimum enzymatic substrate was CMC.
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SONG Lili,WEN Ge,HUO Shanhao,et al. Screening and identification of a cellulase-producing bacterium from distiller′s grains and the enzymatic property[J]. Food and Fermentation Industries, 2020, 46(7): 43-49.
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2020, Vol. 46 
