Abstract: The adipate synthesis pathway from Thermobifida fusca B6 was transformed into Saccharomyces cerevisiae for the first time and succeeded to synthesis adipic acid from glucose. Three shuttle plasmids harboring the pathway genes were combined with suitable constitutive promoters and terminators and introduced into S. cerevisiae. The titer, 3.39 mg/L, was obtained by fermentation of AA-1 strain in YPD medium. To enhance the acceleration of succinyl-CoA from TCA cycle for adipic acid synthesis, the LSC1 gene was deleted. However, it failed to improve adipic acid titer. The relationship between adipic acid titer with the biomass and by-products ethanol was studied. Besides, the yield of ethanol and adipic acid was studied by changing the initial concentration of the carbon source. The results provided a reference for further improving the yield of adipic acid and reducing the Crabtree effect of S. cerevisiae.
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