Abstract: In order to promote the industrial production of β-carotene, carotenoid-related genes were cloned from Sporidiobolus pararoseus and integrated into the genome of Pichia pastoris and a carotenoid-producing strain Pp-EYBI was constructed. Meanwhile, the gene copy number of the rate-limiting enzyme for β-carotene synthesis was optimized and the gene of MVA rate-limiting enzyme was over-expressed to enhance the supply of precursor materials and the flux to β-carotene synthesis. The strain Pp-EYBI+(YB)3H was finally obtained, which yielded as high as 3.7 mg/g (dry cell weight) β-carotene. This lays a foundation for the industrial production of β-carotene by P. pastoris.
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2020, Vol. 46 
