Abstract: A uric acid oxidase-producing strain was screened from the human intestinal tract and identified as Proteus penneri JC-5 by morphological observation and molecular biology (16S rDNA sequencing). The enzymatic properties of the uric acid enzyme produced by JC-5 were studied. According to the experimental results, the optimum temperature of the enzyme was 35 ℃, and the thermal stability was poor; the optimum pH was 8.0, and it had better stability under weak alkaline conditions; Ca2+ and Mn2+ had activation effect on the enzyme, and Ca2+ showed better activation than Mn2+; Fe2+, K+, Mg2+, Cu2+, Zn2+ showed inhibitory effects on the enzyme activity.
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