Abstract: A uric acid oxidase-producing strain was screened from the human intestinal tract and identified as Proteus penneri JC-5 by morphological observation and molecular biology (16S rDNA sequencing). The enzymatic properties of the uric acid enzyme produced by JC-5 were studied. According to the experimental results, the optimum temperature of the enzyme was 35 ℃, and the thermal stability was poor; the optimum pH was 8.0, and it had better stability under weak alkaline conditions; Ca2+ and Mn2+ had activation effect on the enzyme, and Ca2+ showed better activation than Mn2+; Fe2+, K+, Mg2+, Cu2+, Zn2+ showed inhibitory effects on the enzyme activity.
FINI M A,STENMARK K R. Pegloticase and lowering blood pressure in refractory gout; is it uric acid or hydrogen peroxide?[J]. European Journal of Internal Medicine,2019,69:e11-e12.
[3]
ZHANG Ran,GAO Shijue,ZHU Chunyan,et al. Characterization of a novel alkaline Arxula adeninivorans urate oxidase expressed in Escherichia coli and its application in reducing uric acid content of food.[J]. Food chemistry,2019,293:254-262.
[4]
ZHUANG Miaomiao,ZHANG Yanwen,ZHOU Shaohong,et al. Uricase-containing coacervate microdroplets as enzyme active membrane-free protocells for detoxification of uric acid in serum[J]. Chemical communications ,2019,55(92):13 880-13 883.
[5]
IMANI M, SHAHMOHAMADNEJAD S. Recombinant production of Aspergillus Flavus uricase and investigation of its thermal stability in the presence of raffinose and lactose[J]. 3 Biotech, 2017, 7(3):201.
[6]
SORENSEN L B. Role of the intestinal tract in the elimination of uric acid[J]. Arthritis & Rheumatism:Official Journal of the American College of Rheumatology,1965,8(4):694-706.
[7]
LEY R E, PETERSON DA, GORDON J I. Ecological and evolutionary forces shaping microbial diversity in the human intestine[J]. Cell,2006,124(4):837-848.
DRZEWIECKA D. Significance and roles of proteus spp. bacteria in natural environments[J]. Microbial Ecology, 2016, 72(4):741-758.
[15]
SINGH T A, DEVI K R, AHMED G, et al. Microbial and endogenous origin of fibrinolytic activity in traditional fermented foods of Northeast India[J]. Food Research International, 2014, 55: 356-362.
[16]
CANTÁN R, SÓNCHEZ-MORENO M P,REILLY MIM. Proteus penneri[J]. Enfermedades Infecciosas Y Microbiología clínica. 2006,24(S1):8-13.
[17]
MOBLEY H L T, JONES B D, PENNER J L. Urease activity of Proteus penneri[J]. Journal of Clinical Microbiology, 1988, 25(12):2 302-2 305.
[18]
郑丹. 尿毒症患者肠道细菌对小分子尿毒素的分解作用[D]. 长沙:中南大学,2010.
[19]
PESSION A, MELCHIONDA F, CASTELLINI C. Pitfalls, prevention, and treatment of hyperuricemia during tumor lysis syndrome in the era of rasburicase (recombinant urate oxidase)[J]. Biologics Targets & Therapy, 2008, 2(1):129-141.
[20]
PATTE C, SAKIROGLU O, SOMMELET D. European experience in the treatment of hyperuricemia[J]. Seminars in Hematology, 2001, 38(Suppl 10):9-12.
[21]
PATEL K S, LAU J E, ZEMBILLAS A S, et al. Single 4.5 mg fixed-dose of rasburicase for hyperuricemia associated with tumor lysis syndrome[J]. Journal of Oncology Pharmacy Practice, 2017,23(5):333-337.
[22]
KAI L, MA X H, ZHOU X L, et al. Purification and characterization of a thermostable uricase from Microbacterium sp. strain ZZJ4-1[J]. World Journal of Microbiology & Biotechnology, 2008, 24(3):401-406.
2020, Vol. 46 
