Abstract: The presence of Candida albicans in dental plaque with Streptococcus mutans enhances the virulence leading to the onset of rampant caries which is similar to early childhood caries (ECC). In order to verify the inhibitory effect of Lactobacillus plantarum CCFM8724 on the dual biofilm which infected by S. mutans and C. albicans at different time points in vitro, the treated biofilms were assessed for crystal violet staining, anthrone-sulfuric method and colony forming unit (CFU) counting. The live/dead bacteria and structure of biofilm were observed by laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Finally, the decalcification of saliva-coated hydroxyapatite discs (SHA) after culture with CCFM8724 and the tolerance of lysozyme were determined. The results showed that L. plantarum CCFM8724 could significantly reduce the biomass of dual biofilm, the production of water-insoluble extracellular polysaccharide (EPS) and the CFU of pathogens. It also reduced the viability of biofilm and destroyed the structure of biofilm. Moreover, it alleviated the decalcification of SHA with the tolerance concentration to lysozyme 1.6-2.0 mg/mL. It is suggested that CCFM8724 has the potential to be used as an oral probiotic for caries prevention.
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