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食品与发酵工业  2020, Vol. 46 Issue (11): 85-91    DOI: 10.13995/j.cnki.11-1802/ts.023556
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
燕麦β-葡聚糖及其寡糖对肠道菌群结构和代谢的影响
王如月1, 余讯2, 徐静静1, 朱莉3, 詹晓北1*, 张洪涛1
1(工业生物技术教育部重点实验室(江南大学),江苏 无锡,214122)
2(南京医科大学附属无锡第二医院,江苏 无锡,214002)
3(无锡格莱克斯生物科技有限公司,江苏 无锡,214125)
Effects of oat β-glucan and its oligosaccharides on composition and metabolismof intestinal microorganisms
WANG Ruyue1, YU Xun2, XU Jingjing1, ZHU Li3, ZHAN Xiaobei1*, ZHANG Hongtao1
1(Key Laboratory of Industrial Biotechnology, Jiangnan University, Wuxi 214122, China)
2(Wuxi Second People’s Hospital Affiliated to Nanjing Medical University, Wuxi 214002, China)
3(Wuxi Glycose Biotechnology Co., Ltd., Wuxi 214125, China)
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摘要 燕麦β-葡聚糖(oats β-glucans, OGs)及其水解寡糖(oats β-glucan oligosaccharides, OGOs)被认为是具有潜在益生元特性的功能性物质。从燕麦麸皮中提取得到分子质量为1.34×105 Da的β-葡聚糖(OGs),并通过OGs酸水解得到聚合度范围为3~11的β-葡寡糖(OGOs)。分别以OGs和OGOs为培养基碳源,进行健康人和2型糖尿病人来源粪便菌群的体外厌氧发酵,研究OGs和OGOs对不同来源肠道菌群结构和代谢的影响。结果显示,健康人和2型糖尿病人粪便菌群均可以较好地利用OGs和OGOs,在经过48 h发酵后利用率均可以达到93%以上。以OGs和OGOs为碳源对健康人和2型糖尿病人粪便菌群发酵48 h后,发酵液pH均显著降低,乙酸和丙酸含量均显著增加。相比于OGOs,以OGs为碳源发酵粪便菌群48 h后,粪便菌群中产丁酸菌相对总细菌的含量显著提高(P<0.05),相应地,发酵液中丁酸含量显著增加(P<0.05);与OGs相比,以OGOs为碳源则显著增加了粪便菌群中乳酸杆菌和双歧杆菌的相对含量(P<0.05),并且明显增加了发酵液中乙酸和丙酸含量。研究结果显示,OGs和OGOs对肠道菌群的组成和代谢有不同的改善作用,OGs和OGOs可能可以有针对性地改善2型糖尿病人肠道菌群的结构和相关代谢。
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王如月
余讯
徐静静
朱莉
詹晓北
张洪涛
关键词:  β-葡聚糖  β-葡寡糖  肠道微生物  2型糖尿病  短链脂肪酸(short chain fatty acids, SCFA)  体外发酵    
Abstract: Oat β-glucans (OGs) and the resultant oligosaccharides (oats β-glucan oligosaccharides, OGOs) are considered to be functional components with potential prebiotic properties. OGs with a molecular weight of 1.34×105 Da was extracted from oat bran, and OGOs with polymerization degree ranging from 3 to 11, were obtained from acid hydrolysis of OGs. Using OGs and OGOs respectively as the carbon source, in vitro anaerobic fermentation was conducted using fecal flora from healthy people or type 2 diabetic patients. The effects of OGs and OGOs on composition and metabolism of intestinal flora from different sources were studied. The results showed that both fecal floras could make good use of OGs and OGOs, and the utilization rate reached over 93% after 48 h fermentation. The pH value of the fermentation broth significantly decreased, and the content of acetic acid and propionic acid significantly increased after 48 h fermentation. Compared with OGOs, the ratio of butyrate-producing bacteria significantly increased (P<0.05) using OGs as carbon source, leading to a significantly increased (P<0.05) butyric acid content in the fermentation broth. Compared with OGs, using OGOs as a carbon source significantly increased the relative contents of Lactobacillus spp. and Bifidobacterium spp. in the fecal flora (P<0.05). Also, the content of acetic acid and propionic acid was significantly increased in the fermentation broth. Based on the results, OGs and OGOs have different effects on the composition and metabolism of the intestinal flora. And OGs and OGOs may specifically improve the composition and related metabolism of the intestinal flora in type 2 diabetic individuals.
Key words:  β-glucans    β-glucan oligosaccharides    intestinal microorganisms    type 2 diabetes    short chain fatty acids(SCFA)    in vitro fermentation
收稿日期:  2020-02-08                出版日期:  2020-06-15      发布日期:  2020-06-24      期的出版日期:  2020-06-15
基金资助: 十三五国家重点研发技术(2017YFD0400302)
作者简介:  硕士研究生(詹晓北教授为通讯作者,E-mail:xbzhan@yahoo.com)
引用本文:    
王如月,余讯,徐静静,等. 燕麦β-葡聚糖及其寡糖对肠道菌群结构和代谢的影响[J]. 食品与发酵工业, 2020, 46(11): 85-91.
WANG Ruyue,YU Xun,XU Jingjing,et al. Effects of oat β-glucan and its oligosaccharides on composition and metabolismof intestinal microorganisms[J]. Food and Fermentation Industries, 2020, 46(11): 85-91.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.023556  或          http://sf1970.cnif.cn/CN/Y2020/V46/I11/85
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