Biofilm-forming ability of Bifidobacterium and the correlation with cellsurface characteristics
ZHU Ruyi1, HANG Feng2, ZHANG Hao1,2, LI Yuankun3, ZHAO Jianxin1, CHEN Wei1, LU Wenwei1,2*
1(School of Food Science and Technology, Jiangnan University, Wuxi 214122, China) 2((Yangzhou) Institute of Food Biotechnology, Jiangnan University, Yangzhou 225004, China) 3(Department of Microbiology & Immunology, Yong Loo Lin School of Medicine, National University of Singapore,119077, Singapore)
Abstract: To explore the regularity and characteristics of biofilm formation of Bifidobacterium, the biofilm-forming ability of 114 strains distributing in six species of Bifidobacterium, including B. longum, B. breve, B. animalis, B. bifidum, B. adolescentis, and B. pseudocatenulatum, was analyzed by microplate assay. The results showed that according to the absorbance, Bifidobacterium was divided into three types: non-biofilm formers (OD600 nm≤1), weak biofilm formers (1<OD600 nm<3) and strong biofilm formers (OD600 nm≥ 3). All strains of B. bifidum were strong biofilm formers, while B.longum strains were not strong biofilm formers, 57.90% of which were non-biofilm formers. The biofilm-forming ability of other Bifidobacterium was different, including all three types of strains. It was found that the biofilm structures of B.animalis and B.bifidum were mushroom-shaped and all of other Bifidobacterium were flat using scanning electron microscopy, and the biofilms of B.longum and B.bifidum contained a higher proportion of viable bacteria using confocal laser scanning microscopy. The effect of surface properties of Bifidobacterium on the biofilm formation was performed using the adhesion of bacteria to the solvent. The results showed that the biofilm-forming ability of B. pseudocatenulatum was only related to the Lewis acid properties, B. breve, B. animalis and B. adolescentis were positively correlated with the surface hydrophobicity and Lewis acid properties, but there was no correlation between surface properties and biofilm formation for B. longum. The strains of B. bifidum with strong biofilm-forming ability, which exhibited high hydrophobicity and Lewis acid properties, had no relation between its surface properties to its biofilm-forming ability. In summary, the biofilm-forming characteristics of Bifidobacterium exerted species-specific and strain-specific effects and related to the surface characteristics.
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