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食品与发酵工业  2020, Vol. 46 Issue (11): 277-282    DOI: 10.13995/j.cnki.11-1802/ts.023619
  分析与检测 本期目录 | 过刊浏览 | 高级检索 |
基于未修饰的丝网印刷电极测定辣椒碱
李韦琴, 江丰, 曹伟伟, 吴婷, 潘思轶, 徐晓云*
(华中农业大学 食品科学技术学院,湖北 武汉,430070)
Determination of capsaicin based on unmodified screen-printed electrode
LI Weiqin, JIANG Feng, CAO Weiwei, WU Ting, PAN Siyi, XU Xiaoyun*
(College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)
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摘要 建立了一种未修饰的丝网印刷电极测定辣椒碱的电化学检测方法。采用循环伏安法,通过单因素实验研究了乙醇体积分数、pH、KCl浓度和电解质溶液种类对该种丝网印刷电极测定辣椒碱的影响;以稳定性、精密度、检测限和加标回收率等为指标,检测该方法测定辣椒碱的可行性。结果表明,以未修饰的丝网印刷电极检测辣椒碱的适宜条件为:乙醇体积分数10%、pH=1、KCl浓度80 mmol/L、3种电解质溶液Britton-Robison、CH3COOH-CH3COONa、NaH2PO4-Na2HPO4均适宜。辣椒碱氧化峰电流值与其浓度在0.80~107.72 μmol/L范围内呈较好的线性关系,检出限为0.13 μmol/L。此外,该测定方法具有较好的稳定性、精密度和加标回收率,且与HPLC的测定方法结果一致,可作为辣椒碱的快速测定方法。
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李韦琴
江丰
曹伟伟
吴婷
潘思轶
徐晓云
关键词:  辣椒碱  电化学方法  丝网印刷电极  循环伏安法  高效液相色谱法    
Abstract: An electrochemical method for the determination of capsaicin was established using an unmodified screen-printed electrode. The effects of ethanol ratio, electrolyte solution variety, pH value and KCl concentration on the determination of capsaicin by cyclic voltammetry using the screen-printed electrode were studied. The feasibility of the method was tested by stability, precision, detection limit and recovery. Results showed that the optimal parameters for the determination of capsaicin by using this unmodified screen-printed electrode were as follows: 10% ethanol, pH 1 and 80 mmol/L KCl, and Britton-Robison, CH3COOH—CH3COONa and NaH2PO4—Na2HPO4 electrolyte solutions were all suitable for the determination of capsaicin. Linear calibration curve for capsaicin in the range of 0.80~107.72 μmol/L was obtained with a detection limit of 0.13 μmol/L. In addition, the method was of good stability, precision and recovery, which was consistent with the determination of capsaicin by HPLC. Therefore, this method could be used for the rapid determination of capsaicin.
Key words:  capsaicin    electrochemical determination    screen-printed electrode    cyclic voltammetry    high performance liquid chromatography(HPLC)
收稿日期:  2020-02-13                出版日期:  2020-06-15      发布日期:  2020-06-24      期的出版日期:  2020-06-15
基金资助: 湖北省农业科技创新团队—“湖北特色农产品深加工关键技术装备与产业化示范”(2016-620-000-001-044)
作者简介:  硕士研究生(徐晓云教授为通讯作者,E-mail:xuxiaoyun@mail.hzau.edu.cn)
引用本文:    
李韦琴,江丰,曹伟伟,等. 基于未修饰的丝网印刷电极测定辣椒碱[J]. 食品与发酵工业, 2020, 46(11): 277-282.
LI Weiqin,JIANG Feng,CAO Weiwei,et al. Determination of capsaicin based on unmodified screen-printed electrode[J]. Food and Fermentation Industries, 2020, 46(11): 277-282.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.023619  或          http://sf1970.cnif.cn/CN/Y2020/V46/I11/277
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