Abstract: Vegetable protein beverages are popular among consumers because of their rich nutrients. However, adulteration has increased year by year due to the pursuit of profits. In response to this phenomenon, three positive plasmid molecules for detection were constructed in this paper. Three plant constituents of positive plasmids (walnut, peanut and soybean) were constructed, the specificity and sensitivity of three positive plasmids were verified, and the effects of different PCR premixed liquid on the real-time fluorescent quantitative PCR reaction were compared and analyzed. Results showed that the constructed positive plasmid molecules of walnut, peanut and soybean had strong specificity and high sensitivity, and the test sensitivity could reach 10-5 ng/μL. The amplification efficiency of the standard curve is 93.299%, 123.623% and 132.975%, respectively, and the correlation coefficients R2 are all above 0.99. In summary, the three commercial PCR premixed liquid have high sensitivity, which could meet the requirements of routine detection of walnuts, peanuts and soybeans. It is of great significance for the identification of plant-derived components.
翟清燕,任易婕,郑世超,等. 三种植物源性成分阳性质粒的构建及检测方法的验证[J]. 食品与发酵工业, 2020, 46(17): 237-241.
ZHAI Qingyan,REN Yijie,ZHENG Shichao,et al. Verification of detection method and construction of plasmid molecule for three plant-derived components[J]. Food and Fermentation Industries, 2020, 46(17): 237-241.