1(Technology Innovation Center for Exploitation of Marine Biological Resources,Third Institute of Oceanography,Ministry of Natural Resources,Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources,Xiamen 361005,China); 2(College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China)
Abstract: In this paper,the main components,molecular weight distribution and amino acid composition of collagen peptide chelated zinc from puffer skin were analyzed and compared.Its toxicological safety was preliminarily evaluated by acute toxicity test.The results showed that the protein content of collagen peptide chelated zinc from pufferfish skin was (82.04±0.68)%,and the zinc content was (12.86±1.24)%.The molecular weight of collagen peptides from puffer skin was mainly concentrated around 600 Da.Amino acid analysis indicated collagen peptide chelated zinc from puffer skin contained rich glycine,hydroxyproline,aspartic acid and glutamate.The MALDI-TOF mass spectrometry showed the generation of the peptide-zinc chelate.Through the acute toxicity test,the LD50 of collagen peptide chelated zinc from puffer skin was 6.847 9 g/kg,and the 95% confidence limit was 6.122 4~7.676 5 g/kg.According to the acute toxicity dose grading standard,the collagen peptide chelated zinc from puffer skin was actually non-toxic.
郭洪辉,陈晖,俞云,等. 河豚鱼皮胶原肽螯合锌的组成与急性毒性研究[J]. 食品与发酵工业, 2020, 46(23): 41-46.
GUO Honghui,CHEN Hui,YU Yun,et al. Study on the composition and acute toxicity of collagen peptide chelated zinc from puffer skin[J]. Food and Fermentation Industries, 2020, 46(23): 41-46.
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