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食品与发酵工业  2021, Vol. 47 Issue (8): 62-66    DOI: 10.13995/j.cnki.11-1802/ts.024671
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
贾叶, 包斌*, 马明, 魏婷
(上海海洋大学 食品学院,上海,201306)
Effects of enteral nutritional suspension of silkworm pupa protein on gut microbiota in type 2 diabetes mellitus mice
JIA Ye, BAO Bin*, MA Ming, WEI Ting
(School of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China)
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摘要 研究蚕蛹蛋白源肠内营养混悬剂影响二型糖尿病(type 2 diabetes mellities, T2DM)小鼠肠道菌基因组学的代谢差异。采用链脲佐菌素(streptozotocin, STZ)结合高脂饮食建立T2DM小鼠模型。设置对照组(CG)、空白组(BG)、阳性对照组(PG)、蚕蛹蛋白组(SPPG)、蚕蛹蛋白结合乳清蛋白组(LCSSPG),并于造模后给予5周肠内营养支持。每周测量小鼠体重和空腹血糖,在实验第2周、第10周、第15周,每组固定取3只小鼠粪便提取DNA进行16S rRNA V3~V4区测序,并进行口服葡萄糖耐量实验。治疗后,SPPG和PG中Firmicutes/Bacteroidetes的比例有所降低并且接近CG;与BG相比,SPPG和PG中 Lachnospiraceae和Bacteroidaceae菌科相对丰度有所升高。SPPG和LCSSPG中血糖下降率分别为34.17%和27.11%。说明蚕蛹蛋白源肠内营养混悬剂可以对T2DM小鼠肠道菌群的结构和组成成分产生影响。
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关键词:  二型糖尿病  肠道菌群  蛋白质    
Abstract: To study the metabolic differences of silkworm pupa protein-enteral nutritional formula on the gut microbiota of type 2 diabetes mellitus (T2DM) mice, the T2DM model was established by streptozotocin combined with high-fat diet. The female mice were randomly divided into five groups, control group (CG), blank group (BG), positive control group (PG), silkworm pupa protein group (SPPG), silkworm pupa protein combined with whey protein group (LCSSPG). Enteral nutritional support was given for five weeks after the model was made. The body weight and fasting blood glucose of the mice were measured every week. Mice fecal were collected for 16S rRNA V3-V4 region sequencing on the second, tenth and fifteenth week of feeding (3 mice/group), and oral glucose tolerance test was also performed. The relative abundance of Firmicutes/Bacteroidetes was slightly decreased in SPPG and PG, which were close to CG. The relative abundance of Lachnospiraceae and Bacteroidaceae were increased in SPPG and PG. The reduction rates of fasting blood glucose in SPPG and LCSSPG groups were 34.17% and 27.11%, respectively. These results showed that the silkworm pupa protein-enteral nutritional formula could effect the structure and composition of gut microbiota in T2DM mice.
Key words:  type 2 diabetes mellities    gut microbiota    protein
               出版日期:  2021-04-25      发布日期:  2021-05-20      期的出版日期:  2021-04-25
基金资助: 国家自然科学基金(81502955;81750110548);上海市创新行动计划(1749074222500)
作者简介:  硕士研究生(包斌教授为通讯作者,,cn)
贾叶,包斌,马明,等. 蚕蛹蛋白源肠内营养混悬剂对二型糖尿病小鼠肠道菌的影响[J]. 食品与发酵工业, 2021, 47(8): 62-66.
JIA Ye,BAO Bin,MA Ming,et al. Effects of enteral nutritional suspension of silkworm pupa protein on gut microbiota in type 2 diabetes mellitus mice[J]. Food and Fermentation Industries, 2021, 47(8): 62-66.
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