Abstract: To study the metabolic differences of silkworm pupa protein-enteral nutritional formula on the gut microbiota of type 2 diabetes mellitus (T2DM) mice, the T2DM model was established by streptozotocin combined with high-fat diet. The female mice were randomly divided into five groups, control group (CG), blank group (BG), positive control group (PG), silkworm pupa protein group (SPPG), silkworm pupa protein combined with whey protein group (LCSSPG). Enteral nutritional support was given for five weeks after the model was made. The body weight and fasting blood glucose of the mice were measured every week. Mice fecal were collected for 16S rRNA V3-V4 region sequencing on the second, tenth and fifteenth week of feeding (3 mice/group), and oral glucose tolerance test was also performed. The relative abundance of Firmicutes/Bacteroidetes was slightly decreased in SPPG and PG, which were close to CG. The relative abundance ofLachnospiraceae andBacteroidaceae were increased in SPPG and PG. The reduction rates of fasting blood glucose in SPPG and LCSSPG groups were 34.17% and 27.11%, respectively. These results showed that the silkworm pupa protein-enteral nutritional formula could effect the structure and composition of gut microbiota in T2DM mice.
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