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食品与发酵工业  2021, Vol. 47 Issue (6): 26-33    DOI: 10.13995/j.cnki.11-1802/ts.025026
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
短链脂肪酸-酰化淀粉对小鼠抑郁样行为的缓解及机制
朱慧越, 邹仁英, 许梦舒, 王琳琳, 田培郡, 陈卫, 王刚*
(江南大学 食品学院,江苏 无锡,214122)
Short chain fatty acid-acylated starch alleviates depression-like behaviors in mice and its mechanisms
ZHU Huiyue, ZOU Renying, XU Mengshu, WANG Linlin, TIAN Peijun, CHEN Wei, WANG Gang*
(School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
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摘要 大量研究证实“微生物-肠-脑轴”在抑郁症的发生发展中发挥重要作用,而肠道微生物产生的短链脂肪酸(short chain fatty acids,SCFAs),更是参与神经调控作用的关键分子。该研究首次采用SCFA-酰化淀粉在大肠定点释放5类短链脂肪酸,探究其对慢性应激诱导的抑郁症状的缓解及机制。以雄性C57BL/6 J小鼠为研究对象,通过为期5周的慢性不可预知温和应激构建抑郁小鼠模型,通过旷场实验、高架十字迷宫实验和强迫游泳实验对小鼠的抑郁样行为进行评价。同时对小鼠应激激素、神经递质水平及相关酶的表达水平进行检测。结果显示,乙酸、异丁酸和丁酸能在不同程度上减轻小鼠抑郁样行为,其可能机制与SCFAs修复下丘脑-垂体-肾上腺轴反馈调节障碍、恢复5-羟色胺的正常代谢相关。该研究为抑郁症的营养干预提供了新的理论依据。
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朱慧越
邹仁英
许梦舒
王琳琳
田培郡
陈卫
王刚
关键词:  抑郁  短链脂肪酸  酰化淀粉  下丘脑-垂体-肾上腺轴(hypothalamic-pituitary-adrenal axis,HPA 轴)  5-羟色胺    
Abstract: A large number of studies have confirmed that the “microorganism-gut-brain axis” plays an important role in the development of depression, and the short chain fatty acids(SCFAs)produced by gut microbiota are the key molecules involved in neuromodulation. In this study, for the first time, SCFA-acylated starches were used to release 5 types of SCFAs in the large intestine, to explore their alleviation effects on chronic unpredictable mild stress (CUMS)-induced depression in mice and its mechanisms. Using male C57BL/6J mice as the research object, a five-week intervention was used to construct a depressive mice model, and the depression-like behaviors of the mice were evaluated through the open field test, elevated plus maze test and forced swimming test. At the same time, the concentration of stress hormones, neurotransmitters and the gene expression of related enzyme in mice were detected. The results showed that acetic acid, isobutyric acid and butyric acid can alleviate depression-like behaviors in mice to varying degrees. The possible mechanisms are related to SCFAs repairing the hypothalamic-pituitary-adrenal axis feedback regulation disorder and restoring normal metabolism of 5-hydroxytrptamine. This study provides a new theoretical basis for nutrition intervention of depression.
Key words:  depression    short-chain fatty acids    acylated starch    hypothalamic-pituitary-adrenal axis    5-hydroxytrptamine
收稿日期:  2020-07-14      修回日期:  2020-08-31           出版日期:  2021-03-25      发布日期:  2021-04-15      期的出版日期:  2021-03-25
基金资助: 国家自然科学基金面上项目(31972052);江苏高校品牌专业建设工程项目(PPZY2015A052)
作者简介:  学士(王刚副教授为通讯作者,E-mail:wanggang@jiangnan.edu.cn)
引用本文:    
朱慧越,邹仁英,许梦舒,等. 短链脂肪酸-酰化淀粉对小鼠抑郁样行为的缓解及机制[J]. 食品与发酵工业, 2021, 47(6): 26-33.
ZHU Huiyue,ZOU Renying,XU Mengshu,et al. Short chain fatty acid-acylated starch alleviates depression-like behaviors in mice and its mechanisms[J]. Food and Fermentation Industries, 2021, 47(6): 26-33.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.025026  或          http://sf1970.cnif.cn/CN/Y2021/V47/I6/26
[1] HUANG Y, WANG Y, WANG H, et al.Prevalence of mental disorders in China:A cross-sectional epidemiological study [J].The Lancet Psychiatry, 2019, 6(3):211-224.
[2] CRASKE M G, STEIN M B.Anxiety [J].The Lancet, 2016, 388(10 063):3 048-3 059.
[3] ERNY D, ANGELS A L H D,JAITIN D, et al.Host microbiota constantly control maturation and function of microglia in the CNS [J].Nature Neuroscience, 2015, 18(7):965-977.
[4] GAREAU M G, JURY J, MACQUEEN G, et al.Probiotic treatment of rat pups normalises corticosterone release and ameliorates colonic dysfunction induced by maternal separation [J].Gut, 2007, 56(11):1 522-1 528;1 587.
[5] LI B, GUO T, QU F, et al.Matrix-assisted laser desorption ionization:Time of flight mass spectrometryidentified models for detection of ESBL-producing bacterial strains [J].Med Sci Monit Basicres, 2014, 20:176 183.
[6] 陈卫,田培郡,张程程,等.肠道菌群与人体健康的研究热点与进展[J].中国食品学报,2017,17(2):1-9.CHEN W, TIAN P J, ZHANG C C, et al. New progress of research on gut microbiota and human health[J]. Journal of Chinese Institute of Food Science and Technology, 2017, 17(2): 1-9.
[7] KOH A, VADDER F D, KOVATCHEVA-DATCHARY P, et al.From dietary fiber to host physiology:Short-chain fatty acids as key bacterial metabolites[J].Cell, 2016, 165(6):1 332-1 345.
[8] COX H M, TOUGH I R, WOOLSTON A M, et al.Peptide YY is critical for acylethanolamine receptor Gpr119-induced activation of gastrointestinal mucosal responses [J].Cell metabolism, 2010, 11(6):532-542.
[9] REOLON G K, MAURMANN N, WERENICZ A, et al.Posttraining systemic administration of the histone deacetylase inhibitor sodium butyrate ameliorates aging-related memory decline in rats [J].Behavioural brain research, 2011, 221(1):329-332.
[10] TIAN P, WANG G, ZHAO J, et al.Bifidobacterium with the role of 5-hydroxytryptophan synthesis regulation alleviates the symptom of depression and related microbiota dysbiosis [J].J Nutr Biochem, 2019, 66:43-51.
[11] BELLONO N W, BAYRER J R, LEITCH D B, et al.Enterochromaffin cells are gut chemosensors that couple to sensory neural pathways[J].Cell, 2017, 170(1):185-198;216.
[12] CAVAGLIERI C R, NISHIYAMA A, FERNANDES L C, et al.Differential effects of short-chain fatty acids on proliferation and production of pro- and anti-inflammatory cytokines by cultured lymphocytes [J].Life Sciences, 2003, 73(13):1 683-1 690.
[13] FROST G, SLEETH M L, SAHURI-ARISOYLU M, et al.The short-chain fatty acid acetate reduces appetite via a central homeostatic mechanism [J].Nature communications,2014, 5(1):1-11.
[14] 于瀛.乙酰化马铃薯淀粉微晶的制备、性能及应用研究[D].沈阳:沈阳工业大学,2014.YU Y. Study on preparation, property and application of acetylated microcrystalline potato starch[D].Shenyang:Shenyang University of Technology, 2014.
[15] FUKUDA S, TOH H, HASE K, et al.Bifidobacteria can protect from enteropathogenic infection through production of acetate [J].Nature, 2011, 469(7 331):543-547.
[16] WANG L, CEN S, WANG G, et al.Acetic acid and butyric acid released in large intestine play different roles in the alleviation of constipation [J].Journal of Functional Foods, 2020, 69:103 953.
[17] 洪雁,戴栋栋,顾正彪,等.一种制备丁酸酯淀粉的方法:江苏省,CN109400725A[P].2019-03-01.HONG Y, DAI D D, GU Z B, et al. Method for preparing butyrate starch: Jiangsu Province, CN109400725A[P].2019-03-01.
[18] 高静, 罗志刚, 董华壮,等.低取代度木薯淀粉醋酸酯的制备及理化性质的研究[J].现代食品科技, 2008, 24(8):756-758;769.GAO J, LUO Z G, DONG H Z, et al. Study on preparation and properties of cassava acetate starch with low degree of substitution[J]. Modern Food Science and Technology, 2008, 24(8): 756-758;769.
[19] 毛丙永.功能性低聚糖对肠道细菌的影响及机制[D].无锡:江南大学,2015.MAO B Y. Effects of functional oligosaccharides on the gut bacteria and the mechanism[D]. Wuxi:Jiangnan University, 2015.
[20] BUROKAS A, MARTIN-GARCIA E, GUTIERREZ-CUESTA J, et al.Relationships between serotonergic and cannabinoid system in depressive-like behavior:A PET study with [11C]-DASB [J].Journal of Neurochemistry, 2014, 130:126-135.
[21] 王维刚,刘震泽,吴文婷,等.小鼠动物实验方法系列专题(七)旷场实验在小鼠行为分析中的应用[J].中国细胞生物学学报,2011, 33(11):1 191-1 196.WANG W G, LIU Z Z, WU W T, et al. The use of open field test in the behavior analysis of mice[J]. Chinese Journal of Cell Biology, 2011, 33(11): 1 191-1 196.
[22] WALF A A, FRYE C A.The use of the elevated plus maze as an assay of anxiety-related behavior in rodents [J].Nature Protocols, 2007, 2(2):322.
[23] TIAN P, O'RIORDAN K J, LEE Y K, et al.Towards a psychobiotic therapy for depression:Bifidobacterium breve CCFM1025 reverses chronic stress-induced depressive symptoms and gut microbial abnormalities in mice [J].2020, 12:100 216.
[24] TIAN P, ZOU R, SONG L, et al.Ingestion of Bifidobacterium longum subspecies infantis strain CCFM687 regulated emotional behavior and the central BDNF pathway in chronic stress-induced depressive mice through reshaping the gut microbiota[J].Food & Function, 2019, 10(11):7 588-7 598.
[25] GUNDERSEN B B, BLENDY J A.Effects of the histone deacetylase inhibitor sodium butyrate in models of depression and anxiety [J].Neuropharmacology, 2009, 57(1):67-74.
[26] 左玲俊,徐俊冕.HPA轴功能与抑郁症[J].中国心理卫生杂志, 2001(2):112-113;109.ZUO L J, XU J M. HPA axis function and depression[J]. Chinese Mental Health Journal, 2001(2):112-113;109.
[27] 赵丹阳.基于肠道微生物对HPA轴的调控作用探讨通腑降浊方治疗IBS-C模型大鼠的机制研究[D].石家庄:河北医科大学, 2018.ZHAO D Y. Researching the mechanism of Tongfujiangzhuo prescription for the treatment of IBS-C based on the regulation effect of intestinal microflorae on HPA axis[D]. Shijiazhuang:Hebei Medical University, 2018.
[28] AIT-BELGNAOUI A, DURAND H, CARTIER C, et al.Prevention of gut leakiness by a probiotic treatment leads to attenuated HPA response to an acute psychological stress in rats [J].Psychoneuroendocrinology, 2012, 37:1 885-1 895.
[29] MARCEL V D W, BOEHME M, LYTE J M, et al.Short-chain fatty acids:Microbial metabolites that alleviate stress-induced brain-gut axis alterations [J].J Physiol, 2018, 596(20):4 923-4 944.
[30] DALILE B, VERVLIET B, BERGONZELLI G, et al.Colon-delivered short-chain fatty acids attenuate the cortisol response to psychosocial stress in healthy men:A randomized, placebo-controlled trial [J].Neuropsychopharmacology, 2020,45(13):2 257-2 266.
[31] GAGLIANO H, DELGADO-MORALES R, SANZ-GARCIA A, et al.High doses of the histone deacetylase inhibitor sodium butyrate trigger a stress-like response [J].Neuropharmacology, 2014, 79:75-82.
[32] REIGSTAD C S, SALMONSON C E, RAINEY J F, et al.Gut microbes promote colonic serotonin production through an effect of short-chain fatty acids on enterochromaffin cells [J].FASEB Journal:Official Publication of the Federation of American Societies for Experimental Biology,2015, 29(4):1 395-1 403.
[33] YANO J M, YU K, DONALDSON G P, et al.Indigenous bacteria from the gut microbiota regulate host serotonin biosynthesis [J].Cell, 2015, 161(2):264-276.
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