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食品与发酵工业  2020, Vol. 46 Issue (24): 190-197    DOI: 10.13995/j.cnki.11-1802/ts.025188
  生产与科研应用 本期目录 | 过刊浏览 | 高级检索 |
丘苑新*, 杨静娴, 马路凯, 何娣
(仲恺农业工程学院 轻工食品学院,广东 广州, 510225)
Extraction and identification of non-fat-soluble antioxidant components in pomelo seeds
QIU Yuanxin*, YANG Jingxian, MA Lukai, HE Di
(College of Food Science and Technology, Zhongkai University of Agriculture Engineering, Guangzhou 510225, China)
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摘要 用70%(体积分数)乙醇提取脱脂柚籽粉,依次用石油醚、二氯甲烷、乙酸乙酯、正丁醇、和水对柚籽乙醇提取物进行萃取,得到6组不同极性萃取物,测定6组萃取物的抗氧化活性,并用超高压液相-高分辨质谱联用技术快速分析有效萃取物的化学成分,对部分化学成分进行结构分析。结果表明,柚籽不同极性萃取物均有一定的抗氧化效果,其中,乙酸乙酯层的抗氧化效果总体优于其他5组,但总还原能力较差。对乙酸乙酯萃取物进行液相色谱-质谱联用分析,共鉴定出33种化合物,其中黄酮及其苷类17种、异戊烯醇脂类脂质化合物4种、酚类化合物7种、香豆素及其衍生物5种。柚籽中含有丰富的抗氧化物质,并且表现出一定的抗氧化活性,可为柚籽作为天然抗氧化剂的开发提供科学依据。
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关键词:  柚籽  不同极性溶剂  抗氧化  超高压液相-高分辨质谱法  活性成分    
Abstract: Ethanol (EE) with 70% were used to extract defatted pomelo seed powder, and then petroleum ether (PEE), dichloromethane (NCE), ethyl acetate (EAE), n-butanol (NBE), and water (WE) were used to extracts were extracted to obtain 6 groups of extraction sites with different polarities. Determined the antioxidant activity of sites. And used UHPLC-Q-Exactive HFMS technology to quickly analyzed the chemical composition of the effective part, identify the structure of some chemical components. The results showed that the different polar parts of pomelo seeds had certain antioxidant effects, and the antioxidant effect of the ethyl acetate layer was generally better than the other five groups. but the total reduction ability was poor. LC-MS analysis of the ethyl acetate site identified 33 compounds, including 17 flavonoids and their glycosides, 4 isopentenyl lipid compounds, 7 phenolic compounds, coumarin and its 5 derivatives. Pomelo seeds are rich in antioxidants and show certain antioxidant activity, which can provide scientific basis for the development of pomelo seeds as natural antioxidants.
Key words:  pomelo seed    different polar solvents    antioxidant    UHPLC-MS    active ingredients
收稿日期:  2020-07-28      修回日期:  2020-09-14           出版日期:  2020-12-25      发布日期:  2021-01-13      期的出版日期:  2020-12-25
基金资助: 梅州市精准扶贫产业建设技术支撑、规划(2018KA1810322)
作者简介:  博士,副教授(本文通讯作者,
丘苑新,杨静娴,马路凯,等. 柚籽非脂溶性抗氧化成分提取及鉴定[J]. 食品与发酵工业, 2020, 46(24): 190-197.
QIU Yuanxin,YANG Jingxian,MA Lukai,et al. Extraction and identification of non-fat-soluble antioxidant components in pomelo seeds[J]. Food and Fermentation Industries, 2020, 46(24): 190-197.
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