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食品与发酵工业  2021, Vol. 47 Issue (6): 208-213    DOI: 10.13995/j.cnki.11-1802/ts.025220
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云南边贸进口即食食品中金黄色葡萄球菌的分离与鉴定
晏明兴1, 陈芸2, 吕廷保2, 关鼎儒3, 李杜慧1, 何俊蓉1, 梁微3, 杨玲春2*, 胡永金1*, 朱仁俊1*
1(云南农业大学 食品科学技术学院,云南 昆明,650000)
2(昆明海关技术中心,云南 昆明,650000)
3(昆明海关,云南 昆明,650000)
Identification and isolation of Staphylococcus aureus in ready-to-eat food imported from Yunnan border trade
YAN Mingxing1, CHEN Yun2, LYU Tingbao2, GUAN Dingru3, LI Duhui1, HE Junrong1, LIANG Wei3, YANG Lingchun2*, HU Yongjin1*, ZHU Renjun1*
1(College of Food Science and Technology, Yunnan Agricultural University, Kunming 650000, China)
2(Kunming Customs Technical Center, Kunming 650000, China)
3(Kunming Customs, Kunming 650000, China)
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摘要 为探索云南边贸进口即食食品中金黄色葡萄球菌的污染状况,对云南5个边境口岸城市采集到的10类459份进口边贸即食食品中的金黄色葡萄球菌进行检验和分离,并对分离出的可疑金黄色葡萄球菌进行生化确认、全自动微生物鉴定以及16S rDNA序列鉴定,同时利用16S rDNA序列构建系统发育树和同源性分析。结果表明,从所采集的样品中检出2份阳性食品,检出率为0.43%,均为糖果和巧克力,占该类食品的3.8%。从2份阳性食品中分离出9株金黄色葡萄球菌菌株,通过16S rDNA系统发育树和遗传进化分析,9株金黄色葡萄球菌不存在明显的样品来源不同遗传进化差异。该研究结果为构建云南边贸进口食品食源性致病菌溯源技术、致病菌安全预警平台提供理论和技术支持。
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晏明兴
陈芸
吕廷保
关鼎儒
李杜慧
何俊蓉
梁微
杨玲春
胡永金
朱仁俊
关键词:  即食食品  进口食品  金黄色葡萄球菌  16S rDNA  细菌鉴定    
Abstract: In order to investigate the contamination of Staphylococcus aureus in the imported ready-to-eat foods from border trade in Yunnan province, 459 samples of 10 categories collected from 5 border port cities were examined for S. aureus. Biochemical identification, automatic microbial identification (VITEK) and 16S rDNA sequence identification were performed for the isolation of suspicious S. aureus. At the same time, the phylogenetic tree and homology analysis were constructed by using 16S rDNA sequence. The results showed that the detection rate of S. aureus was 0.43%, all of which were candy and chocolate, accounting for 3.8% of this category. Nine strains of S. aureus were isolated from two positive foods, according to phylogenetic analysis of 16S rDNA phylogenetic tree and genetic evolution, these S. aureus strains did not show significant genetic and evolutionary differences in different sample sources. The results provide theoretical and technical support for the establishment of foodborne pathogenic bacteria tracing technology and pathogenic bacteria safety-warning platform for border trade food import in Yunnan province.
Key words:  ready-to-eat food    imported food    Staphylococcus aureus    16S rDNA    bacteria identification
收稿日期:  2020-07-30      修回日期:  2020-09-14           出版日期:  2021-03-25      发布日期:  2021-04-15      期的出版日期:  2021-03-25
基金资助: 云南省重点研发计划(2018BC006)
作者简介:  晏明兴硕士研究生和陈芸高级工程师为共同第一作者(杨玲春研究员、胡永金教授和朱仁俊教授为共同通讯作者,E-mail:Lcyang333@sina.com;271988599@qq.com;2421125201@qq.com)
引用本文:    
晏明兴,陈芸,吕廷保,等. 云南边贸进口即食食品中金黄色葡萄球菌的分离与鉴定[J]. 食品与发酵工业, 2021, 47(6): 208-213.
YAN Mingxing,CHEN Yun,LYU Tingbao,et al. Identification and isolation of Staphylococcus aureus in ready-to-eat food imported from Yunnan border trade[J]. Food and Fermentation Industries, 2021, 47(6): 208-213.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.025220  或          http://sf1970.cnif.cn/CN/Y2021/V47/I6/208
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