Abstract: Taking Ribes stenocarpum Maxim as raw material, the antimicrobial activity and mechanism of R. stenocarpum against Bacillus cereus were investigated. The antibacterial activities were evaluated by agar diffusion assay and double dilution method, the inhibition mechanism was investigated by measuring the fluorescence spectra of membrane protein, the activity of alkaline phosphatase, Na+K+-ATPase, respiratory chain dehydrogenase etc. Results showed that R. stenocarpum had significant inhibitory effect on B. cereus, the minimum inhibitory concentration and minimum bactericidal concentration were 3.13 and 6.25 mg/mL respectively. The extract of R. stenocarpum induced the leakage of alkaline phosphatase, protein and nucleotides into the culture medium, indicating damage to the integrity of cell wall and membrane, also changed the conformation of membrane protein. The activities of Na+K+-ATPase, Ca2+Mg2+-ATPase and T-ATPase were increased, the membrane bound ion channels were activated, the ion concentration inside and outside the cell membrane was changed to resist the influence of adverse environment. The activity of respiratory chain dehydrogenase was inhibited by the extract, and respiration and energy metabolism were affected. The results of scanning electron microscope showed that the extract caused serious deformation of B. cereus cells, and the surface of the cells had abnormal bulge and depression, all of which indicated that R. stenocarpum could effectively inhibit the growth and reproduction of B. cereus, it has the potential to be a natural food preservative.
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2021, Vol. 47 
