1(College of Food and Biological Engineering, The Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area's Medicinal Herbs, Chongqing Three Gorges University, Chongqing 404120, China) 2(College of Pharmacy and Chemistry, Dali University, Dali 671000, China)
Abstract: To optimize the amino acid extraction conditions of Fritillaria thunbergii Mig by response surface methodology, a pre-column derivatization-ultra-performance liquid chromatography method was establish to determine the contents of 14 amino acids in Fritillaria thunbergii Mig bulbs before and after planting in different areas. The following conditions were used for the extraction of amino acids from bulbs in Fritillaria thunbergii Mig, which was 8 mL hydrochloric acid (6 mol/L) for ultrasonication 30 min and hydrolysis at 110 ℃ for 20 h, and then derivatized with phenyl isothiocyanate pre-column and followed by the detection by UPLC which used ACQUITY UPLC BEH C18 column (2.1 mm×150 mm, 1.7 μm). Mobile phase A was 0.1 mol/L sodium acetate solution (pH adjusted by glacial acetic acid=6.5)∶ acetonitrile (97∶3), and mobile phase B was acetonitrile∶water (4∶1); followed by gradient elution and the detection wavelength was 254 nm with flow rate 0.2 mL/min. The calibration curves of 14 kinds of amino acids showed good linearity within their test ranges (r ≥0.999 2). The average recoveries were between 95.12% and 101.05%, and the RSDs were less than 3.00% which indicating that the method was accurate and reliable. The amino acid content of Fritillaria thunbergii Mig before and after planting in different producing areas were different. This method finds the optimal conditions for the extraction of amino acids from Fritillaria thunbergii Mig, which can be used for the determination of amino acids in bulbs of Fritillaria thunbergii Mig.
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2021, Vol. 47 
