Abstract: High-throughput sequencing technology was used to analyze the fungal diversity of Meitauza samples collected from Jianli city and Shishou county in Jingzhou area, Hubei province. The results showed that Ascomycota (57.77%), Basidiomycota (33.54%) and Mucormycota (8.00%) were the dominant fungal phyla in the Meitauza from Jingzhou, and the abundance of each fungal phylum in the samples from Jianli and Shishou was not significantly different (P>0.05). Fusarium (34.60%), Trichosporon (12.51%), Talaromyces (12.51%), Actinomucor (7.27%) and Cladosporium (1.55%) were the dominant fungal genera, and the abundance of Fusarium between the two regions was significantly different (P>0.01), with the average relative abundance of 56.74% and 12.45%, respectively. Ten OTUs were found to exist in all samples, of which OTU1366 and OTU963 were identified as Trichosporon and Fusarium, with average relative abundance of 27.50% and 29.08%, respectively. Through cluster analysis, principal component analysis and multivariate analysis of variance, it was found that there were significant differences in fungal community structure between the samples from two regions(P<0.05), and the difference of fungal groups within Shishou was greater than that of Jianli (P<0.05). By LEfSe analysis, it was found that Galactomyces was the group that caused the difference between two regions and its relative abundance in the samples of Jianli and Shishou was 1.46% and 0.62%, respectively. Thus, although there are a large number of core groups, there are significant differences in fungal community structure of Meitauza produced in different counties and cities in Jingzhou area, and the difference is mainly caused by Galactomyces.
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