Abstract: Contamination of foodborne pathogens is one of the important factors affecting food quality and safety. Therefore, strengthening the monitoring of foodborne pathogens is essential to ensure food safety and prevent foodborne diseases. Compared with traditional culture methods, nucleic acid-based molecular detection technology is faster, more sensitive, and more efficient. Isothermal amplification technologies gradually replace variable temperature amplification technologies with the advantages of simple reaction conditions. This paper briefly described the research progress on loop-mediated isothermal amplification, strand displacement amplification, single primer isothermal amplification, rolling circle amplification, and saltatory rolling circle amplification, as well as their applications in rapid detection of foodborne pathogenic bacteria. In addition, this research pointed out the common problems existing in the isothermal amplification reaction, and proposed solutions. Otherwise, the five isothermal amplification techniques were compared, which provide a reference for the rapid on-site detection and screening of foodborne pathogens.
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